1q3f

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[[Image:1q3f.gif|left|200px]]<br /><applet load="1q3f" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1q3f.gif|left|200px]]
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caption="1q3f, resolution 1.9&Aring;" />
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'''Uracil DNA glycosylase bound to a cationic 1-aza-2'-deoxyribose-contianing DNA'''<br />
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{{Structure
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|PDB= 1q3f |SIZE=350|CAPTION= <scene name='initialview01'>1q3f</scene>, resolution 1.9&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> and <scene name='pdbligand=URA:URACIL'>URA</scene>
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|ACTIVITY=
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|GENE= UNG OR DGU OR UNG15 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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}}
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'''Uracil DNA glycosylase bound to a cationic 1-aza-2'-deoxyribose-contianing DNA'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1Q3F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=URA:'>URA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q3F OCA].
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1Q3F is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q3F OCA].
==Reference==
==Reference==
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Electrostatic guidance of glycosyl cation migration along the reaction coordinate of uracil DNA glycosylase., Bianchet MA, Seiple LA, Jiang YL, Ichikawa Y, Amzel LM, Stivers JT, Biochemistry. 2003 Nov 4;42(43):12455-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14580190 14580190]
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Electrostatic guidance of glycosyl cation migration along the reaction coordinate of uracil DNA glycosylase., Bianchet MA, Seiple LA, Jiang YL, Ichikawa Y, Amzel LM, Stivers JT, Biochemistry. 2003 Nov 4;42(43):12455-60. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14580190 14580190]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: udg]]
[[Category: udg]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:35:25 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:32:27 2008''

Revision as of 11:32, 20 March 2008

Image:1q3f.gif


PDB ID 1q3f

Drag the structure with the mouse to rotate
, resolution 1.9Å
Ligands: and
Gene: UNG OR DGU OR UNG15 (Homo sapiens)
Coordinates: save as pdb, mmCIF, xml



Uracil DNA glycosylase bound to a cationic 1-aza-2'-deoxyribose-contianing DNA


Contents

Overview

The DNA repair enzyme uracil DNA glycosylase has been crystallized with a cationic 1-aza-2'-deoxyribose-containing DNA that mimics the ultimate transition state of the reaction in which the water nucleophile attacks the anomeric center of the oxacarbenium ion-uracil anion reaction intermediate. Comparison with substrate and product structures, and the previous structure of the intermediate determined by kinetic isotope effects, reveals an exquisite example of geometric strain, least atomic motion, and electrophile migration in biological catalysis. This structure provides a rare opportunity to reconstruct the detailed structural transformations that occur along an enzymatic reaction coordinate.

Disease

Known diseases associated with this structure: Immunodeficiency with hyper IgM, type 4 OMIM:[191525]

About this Structure

1Q3F is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Electrostatic guidance of glycosyl cation migration along the reaction coordinate of uracil DNA glycosylase., Bianchet MA, Seiple LA, Jiang YL, Ichikawa Y, Amzel LM, Stivers JT, Biochemistry. 2003 Nov 4;42(43):12455-60. PMID:14580190

Page seeded by OCA on Thu Mar 20 13:32:27 2008

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