4faq
From Proteopedia
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| - | [[ | + | ==Structure of Oceanobacillus iheyensis group II intron in the presence of K+, Ca2+ and 5'-exon== |
| + | <StructureSection load='4faq' size='340' side='right' caption='[[4faq]], [[Resolution|resolution]] 3.11Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4faq]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FAQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FAQ FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SPM:SPERMINE'>SPM</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4e8k|4e8k]], [[4e8m|4e8m]], [[4e8n|4e8n]], [[4e8p|4e8p]], [[4e8q|4e8q]], [[4e8r|4e8r]], [[4e8t|4e8t]], [[4e8v|4e8v]], [[4far|4far]], [[4fau|4fau]], [[4faw|4faw]], [[4fax|4fax]], [[4fb0|4fb0]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4faq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4faq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4faq RCSB], [http://www.ebi.ac.uk/pdbsum/4faq PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Group II introns are self-splicing ribozymes that share a reaction mechanism and a common ancestor with the eukaryotic spliceosome, thereby providing a model system for understanding the chemistry of pre-mRNA splicing. Here we report 14 crystal structures of a group II intron at different stages of catalysis. We provide a detailed mechanism for the first step of splicing, we describe a reversible conformational change between the first and the second steps of splicing, and we present the ligand-free intron structure after splicing in an active state that corresponds to the retrotransposable form of the intron. During each reaction, the reactants are aligned and activated by a heteronuclear four-metal-ion center that contains a metal cluster and obligate monovalent cations, and they adopt a structural arrangement similar to that of protein endonucleases. Based on our data, we propose a model for the splicing cycle and show that it is applicable to the eukaryotic spliceosome. | ||
| - | + | Visualizing Group II Intron Catalysis through the Stages of Splicing.,Marcia M, Pyle AM Cell. 2012 Oct 26;151(3):497-507. doi: 10.1016/j.cell.2012.09.033. PMID:23101623<ref>PMID:23101623</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | == | + | <references/> |
| - | + | __TOC__ | |
| - | [[Category: Marcia, M | + | </StructureSection> |
| - | [[Category: Pyle, A M | + | [[Category: Marcia, M]] |
| + | [[Category: Pyle, A M]] | ||
[[Category: Retrotransposition]] | [[Category: Retrotransposition]] | ||
[[Category: Ribozyme]] | [[Category: Ribozyme]] | ||
Revision as of 16:59, 9 December 2014
Structure of Oceanobacillus iheyensis group II intron in the presence of K+, Ca2+ and 5'-exon
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