1q9b
From Proteopedia
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| - | [[Image:1q9b.jpg|left|200px]] | + | [[Image:1q9b.jpg|left|200px]] |
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| - | '''CRYSTAL STRUCTURE ANALYSIS OF Hev b 6.02 (HEVEIN) AT 1.5 ANGSTROMS RESOLUTION''' | + | {{Structure |
| + | |PDB= 1q9b |SIZE=350|CAPTION= <scene name='initialview01'>1q9b</scene>, resolution 1.50Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''CRYSTAL STRUCTURE ANALYSIS OF Hev b 6.02 (HEVEIN) AT 1.5 ANGSTROMS RESOLUTION''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1Q9B is a [ | + | 1Q9B is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Hevea_brasiliensis Hevea brasiliensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q9B OCA]. |
==Reference== | ==Reference== | ||
| - | Insights into a conformational epitope of Hev b 6.02 (hevein)., Reyes-Lopez CA, Hernandez-Santoyo A, Pedraza-Escalona M, Mendoza G, Hernandez-Arana A, Rodriguez-Romero A, Biochem Biophys Res Commun. 2004 Jan 30;314(1):123-30. PMID:[http:// | + | Insights into a conformational epitope of Hev b 6.02 (hevein)., Reyes-Lopez CA, Hernandez-Santoyo A, Pedraza-Escalona M, Mendoza G, Hernandez-Arana A, Rodriguez-Romero A, Biochem Biophys Res Commun. 2004 Jan 30;314(1):123-30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14715255 14715255] |
[[Category: Hevea brasiliensis]] | [[Category: Hevea brasiliensis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: lectin]] | [[Category: lectin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:34:38 2008'' |
Revision as of 11:34, 20 March 2008
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| , resolution 1.50Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE ANALYSIS OF Hev b 6.02 (HEVEIN) AT 1.5 ANGSTROMS RESOLUTION
Overview
Hevein (Hev b 6.02) is a major IgE-binding allergen in natural rubber latex and manufactured products. Both tryptophans (Trp(21) and Trp(23)) of the hevein molecule were chemically modified with BNPS-skatole (2-nitrophenylsulfenyl-3-methyl-3(')-bromoindolenine); derivatized allergen failed to significantly inhibit binding of serum IgE in ELISA assays. Similarly, skin prick tests showed that hevein-positive patients gave no response with the modified allergen. Dot blot experiments carried out with anti-hevein mono- and polyclonal antibodies confirmed the importance of Trp(21) and Trp(23) for antibody-recognition, and demonstrated the specific cross-reactivity of other molecules containing hevein-like domains. We also report the structure of Hev b 6.02 at an extended resolution (1.5A) and compare its surface properties around Trp residues with those of similar regions in other allergens. Overall our results indicate that the central part of the protein, which comprises three aromatic and other acidic and polar residues, constitutes a conformational epitope.
About this Structure
1Q9B is a Single protein structure of sequence from Hevea brasiliensis. Full crystallographic information is available from OCA.
Reference
Insights into a conformational epitope of Hev b 6.02 (hevein)., Reyes-Lopez CA, Hernandez-Santoyo A, Pedraza-Escalona M, Mendoza G, Hernandez-Arana A, Rodriguez-Romero A, Biochem Biophys Res Commun. 2004 Jan 30;314(1):123-30. PMID:14715255
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