1qc8

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[[Image:1qc8.gif|left|200px]]<br /><applet load="1qc8" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1qc8.gif|left|200px]]
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caption="1qc8" />
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'''NMR STRUCTURE OF TAU EXON 10 SPLICING REGULATORY ELEMENT RNA'''<br />
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{{Structure
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|PDB= 1qc8 |SIZE=350|CAPTION= <scene name='initialview01'>1qc8</scene>
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|SITE=
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|LIGAND=
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|ACTIVITY=
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|GENE=
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}}
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'''NMR STRUCTURE OF TAU EXON 10 SPLICING REGULATORY ELEMENT RNA'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1QC8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QC8 OCA].
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1QC8 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QC8 OCA].
==Reference==
==Reference==
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Structure of tau exon 10 splicing regulatory element RNA and destabilization by mutations of frontotemporal dementia and parkinsonism linked to chromosome 17., Varani L, Hasegawa M, Spillantini MG, Smith MJ, Murrell JR, Ghetti B, Klug A, Goedert M, Varani G, Proc Natl Acad Sci U S A. 1999 Jul 6;96(14):8229-34. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10393977 10393977]
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Structure of tau exon 10 splicing regulatory element RNA and destabilization by mutations of frontotemporal dementia and parkinsonism linked to chromosome 17., Varani L, Hasegawa M, Spillantini MG, Smith MJ, Murrell JR, Ghetti B, Klug A, Goedert M, Varani G, Proc Natl Acad Sci U S A. 1999 Jul 6;96(14):8229-34. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10393977 10393977]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Goedert, M.]]
[[Category: Goedert, M.]]
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[[Category: alternative mrna splicing]]
[[Category: alternative mrna splicing]]
[[Category: frontotemporal dementia ftdp-17]]
[[Category: frontotemporal dementia ftdp-17]]
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[[Category: intronic mutations]]
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[[Category: intronic mutation]]
[[Category: stem-loop rna structure]]
[[Category: stem-loop rna structure]]
[[Category: tau gene exon 10]]
[[Category: tau gene exon 10]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:38:14 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:35:41 2008''

Revision as of 11:35, 20 March 2008


PDB ID 1qc8

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NMR STRUCTURE OF TAU EXON 10 SPLICING REGULATORY ELEMENT RNA


Overview

Coding region and intronic mutations in the tau gene cause frontotemporal dementia and parkinsonism linked to chromosome 17. Intronic mutations and some missense mutations increase splicing in of exon 10, leading to an increased ratio of four-repeat to three-repeat tau isoforms. Secondary structure predictions have led to the proposal that intronic mutations and one missense mutation destabilize a putative RNA stem-loop structure located close to the splice-donor site of the intron after exon 10. We have determined the three-dimensional structure of this tau exon 10 splicing regulatory element RNA by NMR spectroscopy. We show that it forms a stable, folded stem-loop structure whose thermodynamic stability is reduced by frontotemporal dementia and parkinsonism linked to chromosome 17 mutations and increased by compensatory mutations. By exon trapping, the reduction in thermodynamic stability is correlated with increased splicing in of exon 10.

About this Structure

1QC8 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

Structure of tau exon 10 splicing regulatory element RNA and destabilization by mutations of frontotemporal dementia and parkinsonism linked to chromosome 17., Varani L, Hasegawa M, Spillantini MG, Smith MJ, Murrell JR, Ghetti B, Klug A, Goedert M, Varani G, Proc Natl Acad Sci U S A. 1999 Jul 6;96(14):8229-34. PMID:10393977

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