1qhw

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[[Image:1qhw.jpg|left|200px]]<br /><applet load="1qhw" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1qhw.jpg|left|200px]]
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caption="1qhw, resolution 2.2&Aring;" />
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'''PURPLE ACID PHOSPHATASE FROM RAT BONE'''<br />
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{{Structure
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|PDB= 1qhw |SIZE=350|CAPTION= <scene name='initialview01'>1qhw</scene>, resolution 2.2&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2]
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|GENE= ACP5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus])
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}}
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'''PURPLE ACID PHOSPHATASE FROM RAT BONE'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1QHW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=FE:'>FE</scene>, <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QHW OCA].
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1QHW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QHW OCA].
==Reference==
==Reference==
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Three-dimensional structure of a mammalian purple acid phosphatase at 2.2 A resolution with a mu-(hydr)oxo bridged di-iron center., Lindqvist Y, Johansson E, Kaija H, Vihko P, Schneider G, J Mol Biol. 1999 Aug 6;291(1):135-47. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10438611 10438611]
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Three-dimensional structure of a mammalian purple acid phosphatase at 2.2 A resolution with a mu-(hydr)oxo bridged di-iron center., Lindqvist Y, Johansson E, Kaija H, Vihko P, Schneider G, J Mol Biol. 1999 Aug 6;291(1):135-47. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10438611 10438611]
[[Category: Acid phosphatase]]
[[Category: Acid phosphatase]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: metal phosphatase]]
[[Category: metal phosphatase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:39:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:37:49 2008''

Revision as of 11:37, 20 March 2008

Image:1qhw.jpg


PDB ID 1qhw

Drag the structure with the mouse to rotate
, resolution 2.2Å
Ligands: , and
Gene: ACP5 (Rattus norvegicus)
Activity: Acid phosphatase, with EC number 3.1.3.2
Coordinates: save as pdb, mmCIF, xml



PURPLE ACID PHOSPHATASE FROM RAT BONE


Overview

The crystal structure of purple acid phosphatase from rat bone has been determined by molecular replacement and the structure has been refined to 2.2 A resolution to an R -factor of 21.3 % (R -free 26.5 %). The core of the enzyme consists of two seven-stranded mixed beta-sheets, with each sheet flanked by solvent-exposed alpha-helices on one side. The two sheets pack towards each other forming a beta-sandwich. The di-iron center, located at the bottom of the active-site pocket at one edge of the beta-sandwich, contains a mu-hydroxo or mu-oxo bridge and both metal ions are observed in an almost perfect octahedral coordination geometry. The electron density map indicates that a mu-(hydr)oxo bridge is found in the metal center and that at least one solvent molecule is located in the first coordination sphere of one of the metal ions. The crystallographic study of rat purple acid phosphatase reveals that the mammalian enzymes are very similar in overall structure to the plant enzymes in spite of only 18 % overall sequence identity. In particular, coordination and geometry of the iron cluster is preserved in both enzymes and comparison of the active-sites suggests a common mechanism for the mammalian and plant enzymes. However, significant differences are found in the architecture of the substrate binding pocket.

About this Structure

1QHW is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

Three-dimensional structure of a mammalian purple acid phosphatase at 2.2 A resolution with a mu-(hydr)oxo bridged di-iron center., Lindqvist Y, Johansson E, Kaija H, Vihko P, Schneider G, J Mol Biol. 1999 Aug 6;291(1):135-47. PMID:10438611

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