1qni

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[[Image:1qni.jpg|left|200px]]<br /><applet load="1qni" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1qni.jpg|left|200px]]
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caption="1qni, resolution 2.40&Aring;" />
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'''CRYSTAL STRUCTURE OF NITROUS OXIDE REDUCTASE FROM PSEUDOMONAS NAUTICA, AT 2.4A RESOLUTION'''<br />
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{{Structure
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|PDB= 1qni |SIZE=350|CAPTION= <scene name='initialview01'>1qni</scene>, resolution 2.40&Aring;
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|SITE= <scene name='pdbsite=CUA:Cua+Center+(Electron+Entry+Gate)'>CUA</scene>, <scene name='pdbsite=CUB:Cua+Center+(Electron+Entry+Gate)'>CUB</scene>, <scene name='pdbsite=CUC:Cua+Center+(Electron+Entry+Gate)'>CUC</scene>, <scene name='pdbsite=CUD:Cua+Center+(Electron+Entry+Gate)'>CUD</scene>, <scene name='pdbsite=CUE:Cua+Center+(Electron+Entry+Gate)'>CUE</scene>, <scene name='pdbsite=CUF:Cua+Center+(Electron+Entry+Gate)'>CUF</scene>, <scene name='pdbsite=CUU:Cuz+Center+(Catalytic+Site)'>CUU</scene>, <scene name='pdbsite=CUV:Cuz+Center+(Catalytic+Site)'>CUV</scene>, <scene name='pdbsite=CUW:Cuz+Center+(Catalytic+Site)'>CUW</scene>, <scene name='pdbsite=CUX:Cuz+Center+(Catalytic+Site)'>CUX</scene>, <scene name='pdbsite=CUY:Cuz+Center+(Catalytic+Site)'>CUY</scene> and <scene name='pdbsite=CUZ:Cuz+Center+(Catalytic+Site)'>CUZ</scene>
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CUA:DINUCLEAR+COPPER+ION'>CUA</scene> and <scene name='pdbligand=CUZ:(MU-4-SULFIDO)-TETRA-NUCLEAR COPPER ION'>CUZ</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''CRYSTAL STRUCTURE OF NITROUS OXIDE REDUCTASE FROM PSEUDOMONAS NAUTICA, AT 2.4A RESOLUTION'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1QNI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Marinobacter_hydrocarbonoclasticus Marinobacter hydrocarbonoclasticus] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=CUA:'>CUA</scene> and <scene name='pdbligand=CUZ:'>CUZ</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=CUA:Cua+Center+(Electron+Entry+Gate)'>CUA</scene>, <scene name='pdbsite=CUB:Cua+Center+(Electron+Entry+Gate)'>CUB</scene>, <scene name='pdbsite=CUC:Cua+Center+(Electron+Entry+Gate)'>CUC</scene>, <scene name='pdbsite=CUD:Cua+Center+(Electron+Entry+Gate)'>CUD</scene>, <scene name='pdbsite=CUE:Cua+Center+(Electron+Entry+Gate)'>CUE</scene>, <scene name='pdbsite=CUF:Cua+Center+(Electron+Entry+Gate)'>CUF</scene>, <scene name='pdbsite=CUU:Cuz+Center+(Catalytic+Site)'>CUU</scene>, <scene name='pdbsite=CUV:Cuz+Center+(Catalytic+Site)'>CUV</scene>, <scene name='pdbsite=CUW:Cuz+Center+(Catalytic+Site)'>CUW</scene>, <scene name='pdbsite=CUX:Cuz+Center+(Catalytic+Site)'>CUX</scene>, <scene name='pdbsite=CUY:Cuz+Center+(Catalytic+Site)'>CUY</scene> and <scene name='pdbsite=CUZ:Cuz+Center+(Catalytic+Site)'>CUZ</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QNI OCA].
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1QNI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Marinobacter_hydrocarbonoclasticus Marinobacter hydrocarbonoclasticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QNI OCA].
==Reference==
==Reference==
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A novel type of catalytic copper cluster in nitrous oxide reductase., Brown K, Tegoni M, Prudencio M, Pereira AS, Besson S, Moura JJ, Moura I, Cambillau C, Nat Struct Biol. 2000 Mar;7(3):191-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10700275 10700275]
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A novel type of catalytic copper cluster in nitrous oxide reductase., Brown K, Tegoni M, Prudencio M, Pereira AS, Besson S, Moura JJ, Moura I, Cambillau C, Nat Struct Biol. 2000 Mar;7(3):191-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10700275 10700275]
[[Category: Marinobacter hydrocarbonoclasticus]]
[[Category: Marinobacter hydrocarbonoclasticus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: mad]]
[[Category: mad]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:41:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:40:08 2008''

Revision as of 11:40, 20 March 2008

Image:1qni.jpg


PDB ID 1qni

Drag the structure with the mouse to rotate
, resolution 2.40Å
Sites: , , , , , , , , , , and
Ligands: , , and
Coordinates: save as pdb, mmCIF, xml



CRYSTAL STRUCTURE OF NITROUS OXIDE REDUCTASE FROM PSEUDOMONAS NAUTICA, AT 2.4A RESOLUTION


Overview

Nitrous oxide (N20) is a greenhouse gas, the third most significant contributor to global warming. As a key process for N20 elimination from the biosphere, N20 reductases catalyze the two-electron reduction of N20 to N2. These 2 x 65 kDa copper enzymes are thought to contain a CuA electron entry site, similar to that of cytochrome c oxidase, and a CuZ catalytic center. The copper anomalous signal was used to solve the crystal structure of N20 reductase from Pseudomonas nautica by multiwavelength anomalous dispersion, to a resolution of 2.4 A. The structure reveals that the CuZ center belongs to a new type of metal cluster, in which four copper ions are liganded by seven histidine residues. N20 binds to this center via a single copper ion. The remaining copper ions might act as an electron reservoir, assuring a fast electron transfer and avoiding the formation of dead-end products.

About this Structure

1QNI is a Single protein structure of sequence from Marinobacter hydrocarbonoclasticus. Full crystallographic information is available from OCA.

Reference

A novel type of catalytic copper cluster in nitrous oxide reductase., Brown K, Tegoni M, Prudencio M, Pereira AS, Besson S, Moura JJ, Moura I, Cambillau C, Nat Struct Biol. 2000 Mar;7(3):191-5. PMID:10700275

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