1qto
From Proteopedia
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| - | [[Image:1qto.jpg|left|200px]] | + | [[Image:1qto.jpg|left|200px]] |
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| - | '''1.5 A CRYSTAL STRUCTURE OF A BLEOMYCIN RESISTANCE DETERMINANT FROM BLEOMYCIN-PRODUCING STREPTOMYCES VERTICILLUS''' | + | {{Structure |
| + | |PDB= 1qto |SIZE=350|CAPTION= <scene name='initialview01'>1qto</scene>, resolution 1.5Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''1.5 A CRYSTAL STRUCTURE OF A BLEOMYCIN RESISTANCE DETERMINANT FROM BLEOMYCIN-PRODUCING STREPTOMYCES VERTICILLUS''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1QTO is a [ | + | 1QTO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_verticillus Streptomyces verticillus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QTO OCA]. |
==Reference== | ==Reference== | ||
| - | The 1.5 A crystal structure of a bleomycin resistance determinant from bleomycin-producing Streptomyces verticillus., Kawano Y, Kumagai T, Muta K, Matoba Y, Davies J, Sugiyama M, J Mol Biol. 2000 Jan 28;295(4):915-25. PMID:[http:// | + | The 1.5 A crystal structure of a bleomycin resistance determinant from bleomycin-producing Streptomyces verticillus., Kawano Y, Kumagai T, Muta K, Matoba Y, Davies J, Sugiyama M, J Mol Biol. 2000 Jan 28;295(4):915-25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10656800 10656800] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Streptomyces verticillus]] | [[Category: Streptomyces verticillus]] | ||
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[[Category: arm-exchange]] | [[Category: arm-exchange]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:42:35 2008'' |
Revision as of 11:42, 20 March 2008
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1.5 A CRYSTAL STRUCTURE OF A BLEOMYCIN RESISTANCE DETERMINANT FROM BLEOMYCIN-PRODUCING STREPTOMYCES VERTICILLUS
Overview
Bleomycin (Bm)-binding protein, designated BLMA, which is a Bm resistance determinant from Bm-producing Streptomyces verticillus, was crystallized in a form suitable for X-ray diffraction analysis. The diffraction intensity data were collected up to a resolution of 1.5 A with a merging R-value of 0.054 at a completeness of 94 %. The BLMA structure, determined by the single isomorphous replacement method including the anomalous scattering effect (SIR-AS) at a resolution of 2.0 A, was refined at 1.5 A resolution. The final R-factor was 19.0 % and R(free) was 22.1 % including 91 water molecules. The crystal packing showed a dimer form, which was generated by arm exchange. The 1.5 A high-resolution experiment allowed an analysis of the side-chain disorder of BLMA. The structural comparison of BLMA with a homologous protein from Streptoalloteichus hindustanus, designated Shble protein, showed that a Ser100-Gly103 loop was farther from the groove, which is a Bm-binding site, in BLMA than in the Shble protein. Furthermore the hydrophobicity of the groove in BLMA is much lower than that in the Shble protein. The structural differences between these proteins may be responsible for the observation that a half-saturating concentration (K(1/2)) of Bm is higher for BLMA than for the Shble protein.
About this Structure
1QTO is a Single protein structure of sequence from Streptomyces verticillus. Full crystallographic information is available from OCA.
Reference
The 1.5 A crystal structure of a bleomycin resistance determinant from bleomycin-producing Streptomyces verticillus., Kawano Y, Kumagai T, Muta K, Matoba Y, Davies J, Sugiyama M, J Mol Biol. 2000 Jan 28;295(4):915-25. PMID:10656800
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