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4gop

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[[Image:4gop.jpg|left|200px]]
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==Structure and Conformational Change of a Replication Protein A Heterotrimer Bound to ssDNA==
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<StructureSection load='4gop' size='340' side='right' caption='[[4gop]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4gop]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Ustilago_maydis_521 Ustilago maydis 521]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GOP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GOP FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">UM04165.1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=237631 Ustilago maydis 521]), UM02579.1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=237631 Ustilago maydis 521]), UM05156.1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=237631 Ustilago maydis 521])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gop FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gop OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gop RCSB], [http://www.ebi.ac.uk/pdbsum/4gop PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Replication protein A (RPA) is the main eukaryotic ssDNA-binding protein with essential roles in DNA replication, recombination, and repair. RPA maintains the DNA as single-stranded and also interacts with other DNA-processing proteins, coordinating their assembly and disassembly on DNA. RPA binds to ssDNA in two conformational states with opposing affinities for DNA and proteins. The RPA-protein interactions are compatible with a low DNA affinity state that involves DNA-binding domain A (DBD-A) and DBD-B but not with the high DNA affinity state that additionally engages DBD-C and DBD-D. The structure of the high-affinity RPA-ssDNA complex reported here shows a compact quaternary structure held together by a four-way interface between DBD-B, DBD-C, the intervening linker (BC linker), and ssDNA. The BC linker binds into the DNA-binding groove of DBD-B, mimicking DNA. The associated conformational change and partial occlusion of the DBD-A-DBA-B protein-protein interaction site establish a mechanism for the allosteric coupling of RPA-DNA and RPA-protein interactions.
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{{STRUCTURE_4gop| PDB=4gop | SCENE= }}
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Structure and conformational change of a replication protein A heterotrimer bound to ssDNA.,Fan J, Pavletich NP Genes Dev. 2012 Oct 15;26(20):2337-47. doi: 10.1101/gad.194787.112. PMID:23070815<ref>PMID:23070815</ref>
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===Structure and Conformational Change of a Replication Protein A Heterotrimer Bound to ssDNA===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_23070815}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[4gop]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Ustilago_maydis_521 Ustilago maydis 521]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GOP OCA].
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</StructureSection>
[[Category: Ustilago maydis 521]]
[[Category: Ustilago maydis 521]]
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[[Category: Jie, F.]]
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[[Category: Jie, F]]
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[[Category: Pavletich, N P.]]
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[[Category: Pavletich, N P]]
[[Category: Dna binding protein-dna complex]]
[[Category: Dna binding protein-dna complex]]
[[Category: Ob fold]]
[[Category: Ob fold]]
[[Category: Ssdna binding]]
[[Category: Ssdna binding]]

Revision as of 09:37, 10 December 2014

Structure and Conformational Change of a Replication Protein A Heterotrimer Bound to ssDNA

4gop, resolution 3.10Å

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