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1r0n
From Proteopedia
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| - | [[Image:1r0n.gif|left|200px]] | + | [[Image:1r0n.gif|left|200px]] |
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| - | '''Crystal Structure of Heterodimeric Ecdsyone receptor DNA binding complex''' | + | {{Structure |
| + | |PDB= 1r0n |SIZE=350|CAPTION= <scene name='initialview01'>1r0n</scene>, resolution 2.60Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= RXRA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), ECR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of Heterodimeric Ecdsyone receptor DNA binding complex''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1R0N is a [ | + | 1R0N is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R0N OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of the heterodimeric ecdysone receptor DNA-binding complex., Devarakonda S, Harp JM, Kim Y, Ozyhar A, Rastinejad F, EMBO J. 2003 Nov 3;22(21):5827-40. PMID:[http:// | + | Structure of the heterodimeric ecdysone receptor DNA-binding complex., Devarakonda S, Harp JM, Kim Y, Ozyhar A, Rastinejad F, EMBO J. 2003 Nov 3;22(21):5827-40. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14592980 14592980] |
[[Category: Drosophila melanogaster]] | [[Category: Drosophila melanogaster]] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
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[[Category: ultraspiracle]] | [[Category: ultraspiracle]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:45:16 2008'' |
Revision as of 11:45, 20 March 2008
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| , resolution 2.60Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | RXRA (Homo sapiens), ECR (Drosophila melanogaster) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Heterodimeric Ecdsyone receptor DNA binding complex
Overview
Ecdysteroids initiate molting and metamorphosis in insects via a heterodimeric receptor consisting of the ecdysone receptor (EcR) and ultraspiracle (USP). The EcR-USP heterodimer preferentially mediates transcription through highly degenerate pseudo-palindromic response elements, resembling inverted repeats of 5'-AGGTCA-3' separated by 1 bp (IR-1). The requirement for a heterodimeric arrangement of EcR-USP subunits to bind to a symmetric DNA is unusual within the nuclear receptor superfamily. We describe the 2.24 A structure of the EcR-USP DNA-binding domain (DBD) heterodimer bound to an idealized IR-1 element. EcR and USP use similar surfaces, and rely on the deformed minor groove of the DNA to establish protein-protein contacts. As retinoid X receptor (RXR) is the mammalian homolog of USP, we also solved the 2.60 A crystal structure of the EcR-RXR DBD heterodimer on IR-1 and found the dimerization and DNA-binding interfaces to be the same as in the EcR-USP complex. Sequence alignments indicate that the EcR-RXR heterodimer is an important model for understanding how the FXR-RXR heterodimer binds to IR-1 sites.
About this Structure
1R0N is a Protein complex structure of sequences from Drosophila melanogaster and Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of the heterodimeric ecdysone receptor DNA-binding complex., Devarakonda S, Harp JM, Kim Y, Ozyhar A, Rastinejad F, EMBO J. 2003 Nov 3;22(21):5827-40. PMID:14592980
Page seeded by OCA on Thu Mar 20 13:45:16 2008
