1r6f

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[[Image:1r6f.jpg|left|200px]]<br /><applet load="1r6f" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1r6f.jpg|left|200px]]
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caption="1r6f, resolution 2.17&Aring;" />
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'''The structure of Yersinia pestis V-antigen, an essential virulence factor and mediator of immunity against plague'''<br />
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{{Structure
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|PDB= 1r6f |SIZE=350|CAPTION= <scene name='initialview01'>1r6f</scene>, resolution 2.17&Aring;
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|SITE=
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|LIGAND=
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|ACTIVITY=
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'''The structure of Yersinia pestis V-antigen, an essential virulence factor and mediator of immunity against plague'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1R6F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R6F OCA].
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1R6F is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R6F OCA].
==Reference==
==Reference==
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The structure of Yersinia pestis V-antigen, an essential virulence factor and mediator of immunity against plague., Derewenda U, Mateja A, Devedjiev Y, Routzahn KM, Evdokimov AG, Derewenda ZS, Waugh DS, Structure. 2004 Feb;12(2):301-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14962390 14962390]
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The structure of Yersinia pestis V-antigen, an essential virulence factor and mediator of immunity against plague., Derewenda U, Mateja A, Devedjiev Y, Routzahn KM, Evdokimov AG, Derewenda ZS, Waugh DS, Structure. 2004 Feb;12(2):301-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14962390 14962390]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Yersinia pestis]]
[[Category: Yersinia pestis]]
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[[Category: coiled-coil]]
[[Category: coiled-coil]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:47:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:47:31 2008''

Revision as of 11:47, 20 March 2008


PDB ID 1r6f

Drag the structure with the mouse to rotate
, resolution 2.17Å
Coordinates: save as pdb, mmCIF, xml



The structure of Yersinia pestis V-antigen, an essential virulence factor and mediator of immunity against plague


Overview

The LcrV protein (V-antigen) is a multifunctional virulence factor in Yersinia pestis, the causative agent of plague. LcrV regulates the translocation of cytotoxic effector proteins from the bacterium into the cytosol of mammalian cells via a type III secretion system, possesses antihost activities of its own, and is also an active and passive mediator of resistance to disease. Although a crystal structure of this protein has been actively sought for better understanding of its role in pathogenesis, the wild-type LcrV was found to be recalcitrant to crystallization. We employed a surface entropy reduction mutagenesis strategy to obtain crystals of LcrV that diffract to 2.2 A and determined its structure. The refined model reveals a dumbbell-like molecule with a novel fold that includes an unexpected coiled-coil motif, and provides a detailed three-dimensional roadmap for exploring structure-function relationships in this essential virulence determinant.

About this Structure

1R6F is a Single protein structure of sequence from Yersinia pestis. Full crystallographic information is available from OCA.

Reference

The structure of Yersinia pestis V-antigen, an essential virulence factor and mediator of immunity against plague., Derewenda U, Mateja A, Devedjiev Y, Routzahn KM, Evdokimov AG, Derewenda ZS, Waugh DS, Structure. 2004 Feb;12(2):301-6. PMID:14962390

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