1r6z
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1r6z.gif|left|200px]] | + | [[Image:1r6z.gif|left|200px]] |
| - | + | ||
| - | '''The Crystal Structure of the Argonaute2 PAZ domain (as a MBP fusion)''' | + | {{Structure |
| + | |PDB= 1r6z |SIZE=350|CAPTION= <scene name='initialview01'>1r6z</scene>, resolution 2.8Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MAL:MALTOSE'>MAL</scene> and <scene name='pdbligand=NI:NICKEL (II) ION'>NI</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= MALE, Argonaute 2 CG7439-PB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= Escherichia coli, Drosophila melanogaster]) | ||
| + | }} | ||
| + | |||
| + | '''The Crystal Structure of the Argonaute2 PAZ domain (as a MBP fusion)''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1R6Z is a [ | + | 1R6Z is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli,_drosophila_melanogaster Escherichia coli, drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R6Z OCA]. |
==Reference== | ==Reference== | ||
| - | The crystal structure of the Argonaute2 PAZ domain reveals an RNA binding motif in RNAi effector complexes., Song JJ, Liu J, Tolia NH, Schneiderman J, Smith SK, Martienssen RA, Hannon GJ, Joshua-Tor L, Nat Struct Biol. 2003 Dec;10(12):1026-32. Epub 2003 Nov 16. PMID:[http:// | + | The crystal structure of the Argonaute2 PAZ domain reveals an RNA binding motif in RNAi effector complexes., Song JJ, Liu J, Tolia NH, Schneiderman J, Smith SK, Martienssen RA, Hannon GJ, Joshua-Tor L, Nat Struct Biol. 2003 Dec;10(12):1026-32. Epub 2003 Nov 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14625589 14625589] |
[[Category: Escherichia coli, drosophila melanogaster]] | [[Category: Escherichia coli, drosophila melanogaster]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 26: | Line 35: | ||
[[Category: rnai]] | [[Category: rnai]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:47:46 2008'' |
Revision as of 11:47, 20 March 2008
| |||||||
| , resolution 2.8Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | MALE, Argonaute 2 CG7439-PB (Escherichia coli, Drosophila melanogaster) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The Crystal Structure of the Argonaute2 PAZ domain (as a MBP fusion)
Overview
RISC, the RNA-induced silencing complex, uses short interfering RNAs (siRNAs) or micro RNAs (miRNAs) to select its targets in a sequence-dependent manner. Key RISC components are Argonaute proteins, which contain two characteristic domains, PAZ and PIWI. PAZ is highly conserved and is found only in Argonaute proteins and Dicer. We have solved the crystal structure of the PAZ domain of Drosophila Argonaute2. The PAZ domain contains a variant of the OB fold, a module that often binds single-stranded nucleic acids. PAZ domains show low-affinity nucleic acid binding, probably interacting with the 3' ends of single-stranded regions of RNA. PAZ can bind the characteristic two-base 3' overhangs of siRNAs, indicating that although PAZ may not be a primary nucleic acid binding site in Dicer or RISC, it may contribute to the specific and productive incorporation of siRNAs and miRNAs into the RNAi pathway.
About this Structure
1R6Z is a Single protein structure of sequence from Escherichia coli, drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
The crystal structure of the Argonaute2 PAZ domain reveals an RNA binding motif in RNAi effector complexes., Song JJ, Liu J, Tolia NH, Schneiderman J, Smith SK, Martienssen RA, Hannon GJ, Joshua-Tor L, Nat Struct Biol. 2003 Dec;10(12):1026-32. Epub 2003 Nov 16. PMID:14625589
Page seeded by OCA on Thu Mar 20 13:47:46 2008
