1r6u
From Proteopedia
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| - | [[Image:1r6u.jpg|left|200px]] | + | [[Image:1r6u.jpg|left|200px]] |
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| - | '''Crystal structure of an active fragment of human tryptophanyl-tRNA synthetase with cytokine activity''' | + | {{Structure |
| + | |PDB= 1r6u |SIZE=350|CAPTION= <scene name='initialview01'>1r6u</scene>, resolution 2.0Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=TYM:TRYPTOPHANYL-5'AMP'>TYM</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Tryptophan--tRNA_ligase Tryptophan--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.2 6.1.1.2] | ||
| + | |GENE= WARS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of an active fragment of human tryptophanyl-tRNA synthetase with cytokine activity''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1R6U is a [ | + | 1R6U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R6U OCA]. |
==Reference== | ==Reference== | ||
| - | Functional and crystal structure analysis of active site adaptations of a potent anti-angiogenic human tRNA synthetase., Yang XL, Guo M, Kapoor M, Ewalt KL, Otero FJ, Skene RJ, McRee DE, Schimmel P, Structure. 2007 Jul;15(7):793-805. PMID:[http:// | + | Functional and crystal structure analysis of active site adaptations of a potent anti-angiogenic human tRNA synthetase., Yang XL, Guo M, Kapoor M, Ewalt KL, Otero FJ, Skene RJ, McRee DE, Schimmel P, Structure. 2007 Jul;15(7):793-805. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17637340 17637340] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: rossmann fold catalytic domain]] | [[Category: rossmann fold catalytic domain]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:47:38 2008'' |
Revision as of 11:47, 20 March 2008
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| , resolution 2.0Å | |||||||
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| Ligands: | and | ||||||
| Gene: | WARS (Homo sapiens) | ||||||
| Activity: | Tryptophan--tRNA ligase, with EC number 6.1.1.2 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of an active fragment of human tryptophanyl-tRNA synthetase with cytokine activity
Contents |
Overview
Higher eukaryote tRNA synthetases have expanded functions that come from enlarged, more differentiated structures that were adapted to fit aminoacylation function. How those adaptations affect catalytic mechanisms is not known. Presented here is the structure of a catalytically active natural splice variant of human tryptophanyl-tRNA synthetase (TrpRS) that is a potent angiostatic factor. This and related structures suggest that a eukaryote-specific N-terminal extension of the core enzyme changed substrate recognition by forming an active site cap. At the junction of the extension and core catalytic unit, an arginine is recruited to replace a missing landmark lysine almost 200 residues away. Mutagenesis, rapid kinetic, and substrate binding studies support the functional significance of the cap and arginine recruitment. Thus, the enzyme function of human TrpRS has switched more to the N terminus of the sequence. This switch has the effect of creating selective pressure to retain the N-terminal extension for functional expansion.
Disease
Known disease associated with this structure: Wolcott-Rallison syndrome OMIM:[604032]
About this Structure
1R6U is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Functional and crystal structure analysis of active site adaptations of a potent anti-angiogenic human tRNA synthetase., Yang XL, Guo M, Kapoor M, Ewalt KL, Otero FJ, Skene RJ, McRee DE, Schimmel P, Structure. 2007 Jul;15(7):793-805. PMID:17637340
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