1r77
From Proteopedia
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| - | [[Image:1r77.gif|left|200px]] | + | [[Image:1r77.gif|left|200px]] |
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| - | '''Crystal structure of the cell wall targeting domain of peptidylglycan hydrolase ALE-1''' | + | {{Structure |
| + | |PDB= 1r77 |SIZE=350|CAPTION= <scene name='initialview01'>1r77</scene>, resolution 1.75Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Interstitial_collagenase Interstitial collagenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.7 3.4.24.7] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of the cell wall targeting domain of peptidylglycan hydrolase ALE-1''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1R77 is a [ | + | 1R77 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_capitis Staphylococcus capitis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R77 OCA]. |
==Reference== | ==Reference== | ||
| - | Cell wall-targeting domain of glycylglycine endopeptidase distinguishes among peptidoglycan cross-bridges., Lu JZ, Fujiwara T, Komatsuzawa H, Sugai M, Sakon J, J Biol Chem. 2006 Jan 6;281(1):549-58. Epub 2005 Oct 28. PMID:[http:// | + | Cell wall-targeting domain of glycylglycine endopeptidase distinguishes among peptidoglycan cross-bridges., Lu JZ, Fujiwara T, Komatsuzawa H, Sugai M, Sakon J, J Biol Chem. 2006 Jan 6;281(1):549-58. Epub 2005 Oct 28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16257954 16257954] |
[[Category: Interstitial collagenase]] | [[Category: Interstitial collagenase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: sh3b domain]] | [[Category: sh3b domain]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:47:47 2008'' |
Revision as of 11:47, 20 March 2008
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| , resolution 1.75Å | |||||||
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| Activity: | Interstitial collagenase, with EC number 3.4.24.7 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the cell wall targeting domain of peptidylglycan hydrolase ALE-1
Overview
ALE-1, a homologue of lysostaphin, is a peptidoglycan hydrolase that specifically lyses Staphylococcus aureus cell walls by cleaving the pentaglycine linkage between the peptidoglycan chains. Binding of ALE-1 to S. aureus cells through its C-terminal 92 residues, known as the targeting domain, is functionally important for staphylolytic activity. The ALE-1-targeting domain belongs to the SH3b domain family, the prokaryotic counterpart of the eukaryotic SH3 domains. The 1.75 angstroms crystal structure of the targeting domain shows an all-beta fold similar to typical SH3s but with unique features. The structure reveals patches of conserved residues among orthologous targeting domains, forming surface regions that can potentially interact with some common features of the Gram-positive cell wall. ALE-1-targeting domain binding studies employing various bacterial peptidoglycans demonstrate that the length of the interpeptide bridge, as well as the amino acid composition of the peptide, confers the maximum binding of the targeting domain to the staphylococcal peptidoglycan. Truncation of the highly conserved first 9 N-terminal residues results in loss of specificity to S. aureus cell wall-targeting, suggesting that these residues confer specificity to S. aureus cell wall.
About this Structure
1R77 is a Single protein structure of sequence from Staphylococcus capitis. Full crystallographic information is available from OCA.
Reference
Cell wall-targeting domain of glycylglycine endopeptidase distinguishes among peptidoglycan cross-bridges., Lu JZ, Fujiwara T, Komatsuzawa H, Sugai M, Sakon J, J Biol Chem. 2006 Jan 6;281(1):549-58. Epub 2005 Oct 28. PMID:16257954
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