4av7
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | [[ | + | ==Structure determination of the double mutant S233Y F250G from the sec- alkyl sulfatase PisA1== |
| + | <StructureSection load='4av7' size='340' side='right' caption='[[4av7]], [[Resolution|resolution]] 3.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4av7]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_sp._dsm_6611 Pseudomonas sp. dsm 6611]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AV7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AV7 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2yhe|2yhe]], [[4axh|4axh]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4av7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4av7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4av7 RCSB], [http://www.ebi.ac.uk/pdbsum/4av7 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A highly enantioselective and stereoselective secondary alkylsulfatase from Pseudomonas sp. DSM6611 (Pisa1) was heterologously expressed in Escherichia coli BL21, and purified to homogeneity for kinetic and structural studies. Structure determination of Pisa1 by X-ray crystallography showed that the protein belongs to the family of metallo-beta-lactamases with a conserved binuclear Zn(2+) cluster in the active site. In contrast to a closely related alkylsulfatase from Pseudomonas aeruginosa (SdsA1), Pisa1 showed a preference for secondary rather than primary alkyl sulfates, and enantioselectively hydrolyzed the (R)-enantiomer of rac-2-octyl sulfate, yielding (S)-2-octanol with inversion of absolute configuration as a result of C-O bond cleavage. In order to elucidate the mechanism of inverting sulfate ester hydrolysis, for which no counterpart in chemical catalysis exists, we designed variants of Pisa1 guided by three-dimensional structure and docking experiments. In the course of these studies, we identified an invariant histidine (His317) near the sulfate-binding site as the general acid for crucial protonation of the sulfate leaving group. Additionally, amino acid replacements in the alkyl chain-binding pocket generated an enzyme variant that lost its stereoselectivity towards rac-2-octyl sulfate. These findings are discussed in light of the potential use of this enzyme family for applications in biocatalysis. DATABASE: The atomic coordinates and structural factors have been deposited in the Protein Data Bank under the accession codes 2YHE (wild type, crystal form I), 4AV7 (double variant Ser233-->Tyr/Ph250-->Gly) and 4AXH (wild type, crystal form II) STRUCTURED DIGITAL ABSTRACT: Pisa1 and Pisa1 bind by x-ray crystallography (View interaction). | ||
| - | + | Structure and mechanism of an inverting alkylsulfatase from Pseudomonas sp. DSM6611 specific for secondary alkyl sulfates.,Knaus T, Schober M, Kepplinger B, Faccinelli M, Pitzer J, Faber K, Macheroux P, Wagner U FEBS J. 2012 Dec;279(23):4374-84. doi: 10.1111/febs.12027. Epub 2012 Nov 7. PMID:23061549<ref>PMID:23061549</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | == | + | __TOC__ |
| - | + | </StructureSection> | |
[[Category: Pseudomonas sp. dsm 6611]] | [[Category: Pseudomonas sp. dsm 6611]] | ||
| - | [[Category: Faber, K | + | [[Category: Faber, K]] |
| - | [[Category: Knaus, T | + | [[Category: Knaus, T]] |
| - | [[Category: Macharaux, P | + | [[Category: Macharaux, P]] |
| - | [[Category: Schober, M | + | [[Category: Schober, M]] |
| - | [[Category: Wagner, U | + | [[Category: Wagner, U]] |
[[Category: Double mutant]] | [[Category: Double mutant]] | ||
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
[[Category: Inverting alkylsulfatase]] | [[Category: Inverting alkylsulfatase]] | ||
Revision as of 07:48, 25 January 2015
Structure determination of the double mutant S233Y F250G from the sec- alkyl sulfatase PisA1
| |||||||||||
