1rh4

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[[Image:1rh4.gif|left|200px]]<br /><applet load="1rh4" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1rh4.gif|left|200px]]
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caption="1rh4, resolution 1.9&Aring;" />
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'''RH4 DESIGNED RIGHT-HANDED COILED COIL TETRAMER'''<br />
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{{Structure
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|PDB= 1rh4 |SIZE=350|CAPTION= <scene name='initialview01'>1rh4</scene>, resolution 1.9&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene> and <scene name='pdbligand=IPA:ISOPROPYL ALCOHOL'>IPA</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''RH4 DESIGNED RIGHT-HANDED COILED COIL TETRAMER'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1RH4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct] with <scene name='pdbligand=ACE:'>ACE</scene>, <scene name='pdbligand=NH2:'>NH2</scene> and <scene name='pdbligand=IPA:'>IPA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1RH4 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb70_1.html Designer Proteins]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RH4 OCA].
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1RH4 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. The following page contains interesting information on the relation of 1RH4 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb70_1.html Designer Proteins]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RH4 OCA].
==Reference==
==Reference==
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High-resolution protein design with backbone freedom., Harbury PB, Plecs JJ, Tidor B, Alber T, Kim PS, Science. 1998 Nov 20;282(5393):1462-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9822371 9822371]
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High-resolution protein design with backbone freedom., Harbury PB, Plecs JJ, Tidor B, Alber T, Kim PS, Science. 1998 Nov 20;282(5393):1462-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9822371 9822371]
[[Category: Designer Proteins]]
[[Category: Designer Proteins]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: de novo design]]
[[Category: de novo design]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:50:50 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:51:33 2008''

Revision as of 11:51, 20 March 2008

Image:1rh4.gif


PDB ID 1rh4

Drag the structure with the mouse to rotate
, resolution 1.9Å
Ligands: , and
Coordinates: save as pdb, mmCIF, xml



RH4 DESIGNED RIGHT-HANDED COILED COIL TETRAMER


Overview

Recent advances in computational techniques have allowed the design of precise side-chain packing in proteins with predetermined, naturally occurring backbone structures. Because these methods do not model protein main-chain flexibility, they lack the breadth to explore novel backbone conformations. Here the de novo design of a family of alpha-helical bundle proteins with a right-handed superhelical twist is described. In the design, the overall protein fold was specified by hydrophobic-polar residue patterning, whereas the bundle oligomerization state, detailed main-chain conformation, and interior side-chain rotamers were engineered by computational enumerations of packing in alternate backbone structures. Main-chain flexibility was incorporated through an algebraic parameterization of the backbone. The designed peptides form alpha-helical dimers, trimers, and tetramers in accord with the design goals. The crystal structure of the tetramer matches the designed structure in atomic detail.

About this Structure

1RH4 is a Protein complex structure of sequences from Synthetic construct. The following page contains interesting information on the relation of 1RH4 with [Designer Proteins]. Full crystallographic information is available from OCA.

Reference

High-resolution protein design with backbone freedom., Harbury PB, Plecs JJ, Tidor B, Alber T, Kim PS, Science. 1998 Nov 20;282(5393):1462-7. PMID:9822371

Page seeded by OCA on Thu Mar 20 13:51:33 2008

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