1gwj
From Proteopedia
(New page: 200px<br /> <applet load="1gwj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gwj, resolution 2.20Å" /> '''MORPHINONE REDUCTAS...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1GWJ is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]] with FMN as [[http://en.wikipedia.org/wiki/ligand ligand]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GWJ OCA]]. | + | 1GWJ is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]] with FMN as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Site: FMN. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GWJ OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: oxido-reducatase]] | [[Category: oxido-reducatase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 08:33:48 2007'' |
Revision as of 06:29, 30 October 2007
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MORPHINONE REDUCTASE
Overview
The crystal structure of the NADH-dependent bacterial flavoenzyme, morphinone reductase (MR) has been determined at 2.2-A resolution in, complex with the oxidizing substrate codeinone. The structure reveals a, dimeric enzyme comprising two 8-fold beta/alpha barrel domains, each bound, to FMN, and a subunit folding topology and mode of flavin-binding similar, to that found in Old Yellow Enzyme (OYE) and pentaerythritol tetranitrate, (PETN) reductase. The subunit interface of MR is formed by interactions, from an N-terminal beta strand and helices 2 and 8 of the barrel domain, and is different to that seen in OYE. The active site structures of MR, OYE, and PETN reductase are highly conserved reflecting the ability of, these enzymes to catalyze "generic" reactions such as the reduction of, ... [(full description)]
About this Structure
1GWJ is a [Single protein] structure of sequence from [Pseudomonas putida] with FMN as [ligand]. Structure known Active Site: FMN. Full crystallographic information is available from [OCA].
Reference
Crystal structure of bacterial morphinone reductase and properties of the C191A mutant enzyme., Barna T, Messiha HL, Petosa C, Bruce NC, Scrutton NS, Moody PC, J Biol Chem. 2002 Aug 23;277(34):30976-83. Epub 2002 Jun 4. PMID:12048188
Page seeded by OCA on Tue Oct 30 08:33:48 2007
