1s21
From Proteopedia
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| - | [[Image:1s21.gif|left|200px]] | + | [[Image:1s21.gif|left|200px]] |
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| - | '''Crystal Structure of AvrPphF ORF2, A Type III Effector from P. syringae''' | + | {{Structure |
| + | |PDB= 1s21 |SIZE=350|CAPTION= <scene name='initialview01'>1s21</scene>, resolution 2.00Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= AvrPphF ORF2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=319 Pseudomonas syringae pv. phaseolicola]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of AvrPphF ORF2, A Type III Effector from P. syringae''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1S21 is a [ | + | 1S21 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_syringae_pv._phaseolicola Pseudomonas syringae pv. phaseolicola]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S21 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structures of the type III effector protein AvrPphF and its chaperone reveal residues required for plant pathogenesis., Singer AU, Desveaux D, Betts L, Chang JH, Nimchuk Z, Grant SR, Dangl JL, Sondek J, Structure. 2004 Sep;12(9):1669-81. PMID:[http:// | + | Crystal structures of the type III effector protein AvrPphF and its chaperone reveal residues required for plant pathogenesis., Singer AU, Desveaux D, Betts L, Chang JH, Nimchuk Z, Grant SR, Dangl JL, Sondek J, Structure. 2004 Sep;12(9):1669-81. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15341731 15341731] |
[[Category: Pseudomonas syringae pv. phaseolicola]] | [[Category: Pseudomonas syringae pv. phaseolicola]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: predominantly beta-strand]] | [[Category: predominantly beta-strand]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:59:24 2008'' |
Revision as of 11:59, 20 March 2008
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| , resolution 2.00Å | |||||||
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| Gene: | AvrPphF ORF2 (Pseudomonas syringae pv. phaseolicola) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of AvrPphF ORF2, A Type III Effector from P. syringae
Overview
The avrPphF locus from Pseudomonas syringae pv. phaseolicola, the causative agent of bean halo-blight disease, encodes proteins which either enhance virulence on susceptible hosts or elicit defense responses on hosts carrying the R1 resistance gene. Here we present the crystal structures of the two proteins from the avrPphF operon. The structure of AvrPphF ORF1 is strikingly reminiscent of type III chaperones from bacterial pathogens of animals, indicating structural conservation of these specialized chaperones, despite high sequence divergence. The AvrPphF ORF2 effector adopts a novel "mushroom"-like structure containing "head" and "stalk" subdomains. The head subdomain possesses limited structural homology to the catalytic domain of bacterial ADP-ribosyltransferases (ADP-RTs), though no ADP-RT activity was detected for AvrPphF ORF2 in standard assays. Nonetheless, this structural similarity identified two clusters of conserved surface-exposed residues important for both virulence mediated by AvrPphF ORF2 and recognition of this effector by bean plants expressing the R1 resistance gene.
About this Structure
1S21 is a Single protein structure of sequence from Pseudomonas syringae pv. phaseolicola. Full crystallographic information is available from OCA.
Reference
Crystal structures of the type III effector protein AvrPphF and its chaperone reveal residues required for plant pathogenesis., Singer AU, Desveaux D, Betts L, Chang JH, Nimchuk Z, Grant SR, Dangl JL, Sondek J, Structure. 2004 Sep;12(9):1669-81. PMID:15341731
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