1s66

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[[Image:1s66.jpg|left|200px]]<br /><applet load="1s66" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1s66.jpg|left|200px]]
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caption="1s66, resolution 1.8&Aring;" />
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'''Crystal structure of heme domain of direct oxygen sensor from E. coli'''<br />
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{{Structure
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|PDB= 1s66 |SIZE=350|CAPTION= <scene name='initialview01'>1s66</scene>, resolution 1.8&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene> and <scene name='pdbligand=OXY:OXYGEN MOLECULE'>OXY</scene>
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|ACTIVITY=
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|GENE= YDDU, B1489 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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}}
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'''Crystal structure of heme domain of direct oxygen sensor from E. coli'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1S66 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=HEM:'>HEM</scene> and <scene name='pdbligand=OXY:'>OXY</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S66 OCA].
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1S66 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S66 OCA].
==Reference==
==Reference==
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Insights into signal transduction involving PAS domain oxygen-sensing heme proteins from the X-ray crystal structure of Escherichia coli Dos heme domain (Ec DosH)., Park H, Suquet C, Satterlee JD, Kang C, Biochemistry. 2004 Mar 16;43(10):2738-46. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15005609 15005609]
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Insights into signal transduction involving PAS domain oxygen-sensing heme proteins from the X-ray crystal structure of Escherichia coli Dos heme domain (Ec DosH)., Park H, Suquet C, Satterlee JD, Kang C, Biochemistry. 2004 Mar 16;43(10):2738-46. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15005609 15005609]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: OXY]]
[[Category: OXY]]
[[Category: crystal structure]]
[[Category: crystal structure]]
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[[Category: dos]]
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[[Category: do]]
[[Category: heme protein]]
[[Category: heme protein]]
[[Category: oxygen sensor]]
[[Category: oxygen sensor]]
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[[Category: pas]]
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[[Category: pa]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:58:23 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:01:01 2008''

Revision as of 12:01, 20 March 2008

Image:1s66.jpg


PDB ID 1s66

Drag the structure with the mouse to rotate
, resolution 1.8Å
Ligands: and
Gene: YDDU, B1489 (Escherichia coli)
Coordinates: save as pdb, mmCIF, xml



Crystal structure of heme domain of direct oxygen sensor from E. coli


Overview

The X-ray crystal structure of the Escherichia coli (Ec) direct oxygen sensor heme domain (Ec DosH) has been solved to 1.8 A using Fe multiple-wavelength anomalous dispersion (MAD), and the positions of Met95 have been confirmed by selenomethionine ((Se)Met) MAD. Ec DosH is the sensing part of a larger two-domain sensing/signaling protein, in which the signaling domain has phosphodiesterase activity. The asymmetric unit of the crystal lattice contains a dimer comprised of two differently ligated heme domain monomers. Except for the heme ligands, the monomer heme domains are identical. In one monomer, the heme is ligated by molecular oxygen (O(2)), while in the other monomer, an endogenous Met95 with S --> Fe ligation replaces the exogenous O(2) ligand. In both heme domains, the proximal ligand is His77. Analysis of these structures reveals sizable ligand-dependent conformational changes in the protein chain localized in the FG turn, the G(beta)-strand, and the HI turn. These changes provide insight to the mechanism of signal propagation within the heme domain following initiation due to O(2) dissociation.

About this Structure

1S66 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Insights into signal transduction involving PAS domain oxygen-sensing heme proteins from the X-ray crystal structure of Escherichia coli Dos heme domain (Ec DosH)., Park H, Suquet C, Satterlee JD, Kang C, Biochemistry. 2004 Mar 16;43(10):2738-46. PMID:15005609

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