1s6j
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1s6j.jpg|left|200px]] | + | [[Image:1s6j.jpg|left|200px]] |
| - | + | ||
| - | '''N-terminal Region of the Ca2+-saturated calcium regulatory domain (CLD) from Soybean Calcium-dependent Protein Kinase-alpha (CDPK)''' | + | {{Structure |
| + | |PDB= 1s6j |SIZE=350|CAPTION= <scene name='initialview01'>1s6j</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= CDPK SK5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3847 Glycine max]) | ||
| + | }} | ||
| + | |||
| + | '''N-terminal Region of the Ca2+-saturated calcium regulatory domain (CLD) from Soybean Calcium-dependent Protein Kinase-alpha (CDPK)''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1S6J is a [ | + | 1S6J is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Glycine_max Glycine max]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S6J OCA]. |
==Reference== | ==Reference== | ||
| - | Solution structure and backbone dynamics of the N-terminal region of the calcium regulatory domain from soybean calcium-dependent protein kinase alpha., Weljie AM, Gagne SM, Vogel HJ, Biochemistry. 2004 Dec 7;43(48):15131-40. PMID:[http:// | + | Solution structure and backbone dynamics of the N-terminal region of the calcium regulatory domain from soybean calcium-dependent protein kinase alpha., Weljie AM, Gagne SM, Vogel HJ, Biochemistry. 2004 Dec 7;43(48):15131-40. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15568805 15568805] |
[[Category: Glycine max]] | [[Category: Glycine max]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 18: | Line 27: | ||
[[Category: ef-hand; helix-loop-helix; calcium-binding; calmodulin superfamily]] | [[Category: ef-hand; helix-loop-helix; calcium-binding; calmodulin superfamily]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:01:08 2008'' |
Revision as of 12:01, 20 March 2008
| |||||||
| Ligands: | |||||||
| Gene: | CDPK SK5 (Glycine max) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
N-terminal Region of the Ca2+-saturated calcium regulatory domain (CLD) from Soybean Calcium-dependent Protein Kinase-alpha (CDPK)
Overview
Ca(2+)-dependent protein kinases (CDPKs) are vital Ca(2+)-signaling proteins in plants and protists which have both a kinase domain and a self-contained calcium regulatory calmodulin-like domain (CLD). Despite being very similar to CaM (>40% identity) and sharing the same fold, recent biochemical and structural evidence suggests that the behavior of CLD is distinct from its namesake, calmodulin. In this study, NMR spectroscopy is employed to examine the structure and backbone dynamics of a 168 amino acid Ca(2+)-saturated construct of the CLD (NtH-CLD) in which almost the entire C-terminal domain is exchange broadened and not visible in the NMR spectra. Structural characterization of the N-terminal domain indicates that the first Ca(2+)-binding loop is significantly more open than in a recently reported structure of the CLD complexed with a putative intramolecular binding region (JD) in the CDPK. Backbone dynamics suggest that parts of the third helix exhibit unusually high mobility, and significant exchange, consistent with previous findings that this helix interacts with the C-terminal domain. Dynamics data also show that the "tether" region, consisting of the first 11 amino acids of CLD, is highly mobile and these residues exhibit distinctive beta-type secondary structure, which may help to position the JD and CLD. Finally, the unusual global dynamic behavior of the protein is rationalized on the basis of possible interdomain rearrangements and the highly variable environments of the C- and N-terminal domains.
About this Structure
1S6J is a Single protein structure of sequence from Glycine max. Full crystallographic information is available from OCA.
Reference
Solution structure and backbone dynamics of the N-terminal region of the calcium regulatory domain from soybean calcium-dependent protein kinase alpha., Weljie AM, Gagne SM, Vogel HJ, Biochemistry. 2004 Dec 7;43(48):15131-40. PMID:15568805
Page seeded by OCA on Thu Mar 20 14:01:08 2008
