4fay
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | [[ | + | ==Crystal structure of a trimeric bacterial microcompartment shell protein PduB with glycerol metabolites== |
| + | <StructureSection load='4fay' size='340' side='right' caption='[[4fay]], [[Resolution|resolution]] 1.56Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4fay]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_reuteri_dsm_20016 Lactobacillus reuteri dsm 20016]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FAY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FAY FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Lreu_1748, PduB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=557436 Lactobacillus reuteri DSM 20016])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fay FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fay OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fay RCSB], [http://www.ebi.ac.uk/pdbsum/4fay PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Lactobacillus reuteri metabolizes two similar three-carbon molecules, 1,2-propanediol and glycerol, within closed polyhedral subcellular bacterial organelles called bacterial microcompartments (metabolosomes). The outer shell of the propanediol-utilization (Pdu) metabolosome is composed of hundreds of mainly hexagonal protein complexes made from six types of protein subunits that share similar domain structures. The structure of the bacterial microcompartment protein PduB has a tandem structural repeat within the subunit and assembles into a trimer with pseudo-hexagonal symmetry. This trimeric structure forms sheets in the crystal lattice and is able to fit within a polymeric sheet of the major shell component PduA to assemble a facet of the polyhedron. There are three pores within the trimer and these are formed between the tandem repeats within the subunits. The structure shows that each of these pores contains three glycerol molecules that interact with conserved residues, strongly suggesting that these subunit pores channel glycerol substrate into the metabolosome. In addition to the observation of glycerol occupying the subunit channels, the presence of glycerol on the molecular threefold symmetry axis suggests a role in locking closed the central region. | ||
| - | + | Substrate channels revealed in the trimeric Lactobacillus reuteri bacterial microcompartment shell protein PduB.,Pang A, Liang M, Prentice MB, Pickersgill RW Acta Crystallogr D Biol Crystallogr. 2012 Dec;68(Pt 12):1642-52. doi:, 10.1107/S0907444912039315. Epub 2012 Nov 9. PMID:23151629<ref>PMID:23151629</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | == | + | __TOC__ |
| - | + | </StructureSection> | |
[[Category: Lactobacillus reuteri dsm 20016]] | [[Category: Lactobacillus reuteri dsm 20016]] | ||
| - | [[Category: Pang, A H | + | [[Category: Pang, A H]] |
| - | [[Category: Pickersgill, R W | + | [[Category: Pickersgill, R W]] |
| - | [[Category: Prentice, M B | + | [[Category: Prentice, M B]] |
[[Category: Bmc domain]] | [[Category: Bmc domain]] | ||
[[Category: Glycerol-binding protein]] | [[Category: Glycerol-binding protein]] | ||
[[Category: Shell protein]] | [[Category: Shell protein]] | ||
Revision as of 17:44, 9 December 2014
Crystal structure of a trimeric bacterial microcompartment shell protein PduB with glycerol metabolites
| |||||||||||
