1sg0

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1sg0.gif|left|200px]]<br /><applet load="1sg0" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:1sg0.gif|left|200px]]
-
caption="1sg0, resolution 1.50&Aring;" />
+
 
-
'''Crystal structure analysis of QR2 in complex with resveratrol'''<br />
+
{{Structure
 +
|PDB= 1sg0 |SIZE=350|CAPTION= <scene name='initialview01'>1sg0</scene>, resolution 1.50&Aring;
 +
|SITE=
 +
|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene> and <scene name='pdbligand=STL:RESVERATROL'>STL</scene>
 +
|ACTIVITY= [http://en.wikipedia.org/wiki/NAD(P)H_dehydrogenase_(quinone) NAD(P)H dehydrogenase (quinone)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.5.2 1.6.5.2]
 +
|GENE= NQO2, NMOR2, BC006096 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
 +
}}
 +
 
 +
'''Crystal structure analysis of QR2 in complex with resveratrol'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
1SG0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=FAD:'>FAD</scene> and <scene name='pdbligand=STL:'>STL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/NAD(P)H_dehydrogenase_(quinone) NAD(P)H dehydrogenase (quinone)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.5.2 1.6.5.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SG0 OCA].
+
1SG0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SG0 OCA].
==Reference==
==Reference==
-
Crystal structure of quinone reductase 2 in complex with resveratrol., Buryanovskyy L, Fu Y, Boyd M, Ma Y, Hsieh TC, Wu JM, Zhang Z, Biochemistry. 2004 Sep 14;43(36):11417-26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15350128 15350128]
+
Crystal structure of quinone reductase 2 in complex with resveratrol., Buryanovskyy L, Fu Y, Boyd M, Ma Y, Hsieh TC, Wu JM, Zhang Z, Biochemistry. 2004 Sep 14;43(36):11417-26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15350128 15350128]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: NAD(P)H dehydrogenase (quinone)]]
[[Category: NAD(P)H dehydrogenase (quinone)]]
Line 27: Line 36:
[[Category: resveratrol]]
[[Category: resveratrol]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:01:06 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:04:23 2008''

Revision as of 12:04, 20 March 2008

Image:1sg0.gif


PDB ID 1sg0

Drag the structure with the mouse to rotate
, resolution 1.50Å
Ligands: , and
Gene: NQO2, NMOR2, BC006096 (Homo sapiens)
Activity: NAD(P)H dehydrogenase (quinone), with EC number 1.6.5.2
Coordinates: save as pdb, mmCIF, xml



Crystal structure analysis of QR2 in complex with resveratrol


Overview

Resveratrol has been shown to have chemopreventive, cardioprotective, and antiaging properties. Here, we report that resveratrol is a potent inhibitor of quinone reductase 2 (QR2) activity in vitro with a dissociation constant of 35 nM and show that it specifically binds to the deep active-site cleft of QR2 using high-resolution structural analysis. All three resveratrol hydroxyl groups form hydrogen bonds with amino acids from QR2, anchoring a flat resveratrol molecule in parallel with the isoalloxazine ring of FAD. The unique active-site pocket in QR2 could potentially bind other natural polyphenols such as flavonoids, as proven by the high affinity exhibited by quercetin toward QR2. K562 cells with QR2 expression suppressed by RNAi showed similar properties as resveratrol-treated cells in their resistance to quinone toxicity. Furthermore, the QR2 knockdown K562 cells exhibit increased antioxidant and detoxification enzyme expression and reduced proliferation rates. These observations could imply that the chemopreventive and cardioprotective properties of resveratrol are possibly the results of QR2 activity inhibition, which in turn, up-regulates the expression of cellular antioxidant enzymes and cellular resistance to oxidative stress.

About this Structure

1SG0 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of quinone reductase 2 in complex with resveratrol., Buryanovskyy L, Fu Y, Boyd M, Ma Y, Hsieh TC, Wu JM, Zhang Z, Biochemistry. 2004 Sep 14;43(36):11417-26. PMID:15350128

Page seeded by OCA on Thu Mar 20 14:04:23 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1sg0"
Personal tools