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1ouz

From Proteopedia

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[[Image:1ouz.png|left|200px]]
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==Crystal structure of a mutant IHF (BetaE44A) complexed with a variant H' Site (T44A)==
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<StructureSection load='1ouz' size='340' side='right' caption='[[1ouz]], [[Resolution|resolution]] 2.41&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ouz]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OUZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OUZ FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ihf|1ihf]]</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ouz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ouz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ouz RCSB], [http://www.ebi.ac.uk/pdbsum/1ouz PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ou/1ouz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Integration host factor (IHF) is a DNA-bending protein that recognizes its cognate sites through indirect readout. Previous studies have shown that binding of wild-type (WT)-IHF is disrupted by a T to A mutation at the center position of a conserved TTR motif in its binding site, and that substitution of betaGlu44 with Ala prevented IHF from discriminating between A and T at this position. We have determined the crystal structures and relative binding affinities for all combinations of WT-IHF and IHF-betaGlu44Ala bound to the WT and mutant DNAs. Comparison of these structures reveals that DNA twist plays a major role in DNA recognition by IHF, and that this geometric parameter is dependent on the dinucleotide step and not on the bound IHF variant.
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{{STRUCTURE_1ouz| PDB=1ouz | SCENE= }}
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Integration host factor: putting a twist on protein-DNA recognition.,Lynch TW, Read EK, Mattis AN, Gardner JF, Rice PA J Mol Biol. 2003 Jul 11;330(3):493-502. PMID:12842466<ref>PMID:12842466</ref>
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===Crystal structure of a mutant IHF (BetaE44A) complexed with a variant H' Site (T44A)===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_12842466}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[1ouz]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OUZ OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:012842466</ref><references group="xtra"/>
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Gardner, J F.]]
[[Category: Gardner, J F.]]

Revision as of 23:29, 28 September 2014

Crystal structure of a mutant IHF (BetaE44A) complexed with a variant H' Site (T44A)

1ouz, resolution 2.41Å

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OCA

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