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1skj

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[[Image:1skj.gif|left|200px]]<br /><applet load="1skj" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1skj.gif|left|200px]]
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caption="1skj, resolution 2.0&Aring;" />
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'''COCRYSTAL STRUCTURE OF UREA-SUBSTITUTED PHOSPHOPEPTIDE COMPLEX'''<br />
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{{Structure
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|PDB= 1skj |SIZE=350|CAPTION= <scene name='initialview01'>1skj</scene>, resolution 2.0&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=UR2:4-[3-CARBOXYMETHYL-3-(4-PHOSPHONOOXY-BENZYL)-UREIDO]-4-[(3-CYCLOHEXYL-PROPYL)-METHYL-CARBAMOYL]BUTYRIC ACID'>UR2</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Transferase Transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.10.1 and 2.7.10.2 2.7.10.1 and 2.7.10.2]
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|GENE=
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}}
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'''COCRYSTAL STRUCTURE OF UREA-SUBSTITUTED PHOSPHOPEPTIDE COMPLEX'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1SKJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rous_sarcoma_virus Rous sarcoma virus] with <scene name='pdbligand=UR2:'>UR2</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Transferase Transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.10.1 and 2.7.10.2 2.7.10.1 and 2.7.10.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SKJ OCA].
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1SKJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rous_sarcoma_virus Rous sarcoma virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SKJ OCA].
==Reference==
==Reference==
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Design, synthesis, and cocrystal structure of a nonpeptide Src SH2 domain ligand., Plummer MS, Holland DR, Shahripour A, Lunney EA, Fergus JH, Marks JS, McConnell P, Mueller WT, Sawyer TK, J Med Chem. 1997 Nov 7;40(23):3719-25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9371236 9371236]
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Design, synthesis, and cocrystal structure of a nonpeptide Src SH2 domain ligand., Plummer MS, Holland DR, Shahripour A, Lunney EA, Fergus JH, Marks JS, McConnell P, Mueller WT, Sawyer TK, J Med Chem. 1997 Nov 7;40(23):3719-25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9371236 9371236]
[[Category: Rous sarcoma virus]]
[[Category: Rous sarcoma virus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: v-src sh2 domain]]
[[Category: v-src sh2 domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:02:29 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:06:08 2008''

Revision as of 12:06, 20 March 2008


PDB ID 1skj

Drag the structure with the mouse to rotate
, resolution 2.0Å
Ligands:
Activity: Transferase, with EC number and 2.7.10.2 2.7.10.1 and 2.7.10.2
Coordinates: save as pdb, mmCIF, xml



COCRYSTAL STRUCTURE OF UREA-SUBSTITUTED PHOSPHOPEPTIDE COMPLEX


Overview

The specific association of an SH2 domain with a phosphotyrosine (pTyr)-containing sequence of another protein precipitates a cascade of intracellular molecular interactions (signals) which effect a wide range of intracellular processes. The nonreceptor tyrosine kinase Src, which has been associated with breast cancer and osteoporosis, contains an SH2 domain. Inhibition of Src SH2-phosphoprotein interactions by small molecules will aid biological proof-of-concept studies which may lead to the development of novel therapeutic agents. Structure-based design efforts have focused on reducing the size and charge of Src SH2 ligands while increasing their ability to penetrate cells and reach the intracellular Src SH2 domain target. In this report we describe the synthesis, binding affinity, and Src SH2 cocrystal structure of a small, novel, nonpeptide, urea-containing SH2 domain ligand.

About this Structure

1SKJ is a Single protein structure of sequence from Rous sarcoma virus. Full crystallographic information is available from OCA.

Reference

Design, synthesis, and cocrystal structure of a nonpeptide Src SH2 domain ligand., Plummer MS, Holland DR, Shahripour A, Lunney EA, Fergus JH, Marks JS, McConnell P, Mueller WT, Sawyer TK, J Med Chem. 1997 Nov 7;40(23):3719-25. PMID:9371236

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