1smp
From Proteopedia
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| - | [[Image:1smp.jpg|left|200px]] | + | [[Image:1smp.jpg|left|200px]] |
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| - | '''CRYSTAL STRUCTURE OF A COMPLEX BETWEEN SERRATIA MARCESCENS METALLO-PROTEASE AND AN INHIBITOR FROM ERWINIA CHRYSANTHEMI''' | + | {{Structure |
| + | |PDB= 1smp |SIZE=350|CAPTION= <scene name='initialview01'>1smp</scene>, resolution 2.30Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Serralysin Serralysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.40 3.4.24.40] | ||
| + | |GENE= INH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=615 Serratia marcescens]), INH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=556 Erwinia chrysanthemi]) | ||
| + | }} | ||
| + | |||
| + | '''CRYSTAL STRUCTURE OF A COMPLEX BETWEEN SERRATIA MARCESCENS METALLO-PROTEASE AND AN INHIBITOR FROM ERWINIA CHRYSANTHEMI''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1SMP is a [ | + | 1SMP is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi] and [http://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SMP OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of a complex between Serratia marcescens metallo-protease and an inhibitor from Erwinia chrysanthemi., Baumann U, Bauer M, Letoffe S, Delepelaire P, Wandersman C, J Mol Biol. 1995 May 5;248(3):653-61. PMID:[http:// | + | Crystal structure of a complex between Serratia marcescens metallo-protease and an inhibitor from Erwinia chrysanthemi., Baumann U, Bauer M, Letoffe S, Delepelaire P, Wandersman C, J Mol Biol. 1995 May 5;248(3):653-61. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7752231 7752231] |
[[Category: Erwinia chrysanthemi]] | [[Category: Erwinia chrysanthemi]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: complex (metalloprotease/inhibitor)]] | [[Category: complex (metalloprotease/inhibitor)]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:07:08 2008'' |
Revision as of 12:07, 20 March 2008
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| , resolution 2.30Å | |||||||
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| Ligands: | and | ||||||
| Gene: | INH (Serratia marcescens), INH (Erwinia chrysanthemi) | ||||||
| Activity: | Serralysin, with EC number 3.4.24.40 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF A COMPLEX BETWEEN SERRATIA MARCESCENS METALLO-PROTEASE AND AN INHIBITOR FROM ERWINIA CHRYSANTHEMI
Overview
The crystal structure of the complex between the 50 kDa metallo-endoproteinase from Serratia marcescens (SMP), a member of the metzincin superfamily, and an inhibitor from Erwinia chrysanthemi (Inh) was solved by molecular replacement using the known structure of SMP, and refined at 2.30 A resolution to a crystallographic R-factor of 0.195. The E. chrysanthemi inhibitor folds into a compact eight-stranded antiparallel beta-barrel of simple up-down topology such as is found for members of the retinol binding protein family. It mainly interacts with the protease via its five N-terminal residues, which insert into the active site cleft, occupying the S' sites. The first N-terminal residue, SerI1, is partially cleaved off by the protease, while SerI2 makes a hydrogen bond with the catalytically active glutamic acid, Glu177, of the protease. Further interactions are made between one face of the inhibitor formed by the strands s3, s4 and s5 and the protease segment 218 to 228, which is located immediately after the characteristic "Met-turn" of the metzincins.
About this Structure
1SMP is a Protein complex structure of sequences from Erwinia chrysanthemi and Serratia marcescens. Full crystallographic information is available from OCA.
Reference
Crystal structure of a complex between Serratia marcescens metallo-protease and an inhibitor from Erwinia chrysanthemi., Baumann U, Bauer M, Letoffe S, Delepelaire P, Wandersman C, J Mol Biol. 1995 May 5;248(3):653-61. PMID:7752231
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