1sr4
From Proteopedia
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| - | [[Image:1sr4.jpg|left|200px]] | + | [[Image:1sr4.jpg|left|200px]] |
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| - | '''Crystal Structure of the Haemophilus ducreyi cytolethal distending toxin''' | + | {{Structure |
| + | |PDB= 1sr4 |SIZE=350|CAPTION= <scene name='initialview01'>1sr4</scene>, resolution 2.0Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=BR:BROMIDE ION'>BR</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= CDTA, HD0902 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=730 Haemophilus ducreyi]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of the Haemophilus ducreyi cytolethal distending toxin''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1SR4 is a [ | + | 1SR4 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Haemophilus_ducreyi Haemophilus ducreyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SR4 OCA]. |
==Reference== | ==Reference== | ||
| - | Assembly and function of a bacterial genotoxin., Nesic D, Hsu Y, Stebbins CE, Nature. 2004 May 27;429(6990):429-33. PMID:[http:// | + | Assembly and function of a bacterial genotoxin., Nesic D, Hsu Y, Stebbins CE, Nature. 2004 May 27;429(6990):429-33. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15164065 15164065] |
[[Category: Haemophilus ducreyi]] | [[Category: Haemophilus ducreyi]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: bacterial]] | [[Category: bacterial]] | ||
[[Category: cell cycle]] | [[Category: cell cycle]] | ||
| - | [[Category: | + | [[Category: cytotoxin]] |
[[Category: deoxyribonuclease i]] | [[Category: deoxyribonuclease i]] | ||
[[Category: dna damage]] | [[Category: dna damage]] | ||
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[[Category: virulence]] | [[Category: virulence]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:08:40 2008'' |
Revision as of 12:08, 20 March 2008
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| , resolution 2.0Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | CDTA, HD0902 (Haemophilus ducreyi) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the Haemophilus ducreyi cytolethal distending toxin
Overview
The tripartite cytolethal distending toxin (CDT) induces cell cycle arrest and apoptosis in eukaryotic cells. The subunits CdtA and CdtC associate with the nuclease CdtB to form a holotoxin that translocates CdtB into the host cell, where it acts as a genotoxin by creating DNA lesions. Here we show that the crystal structure of the holotoxin from Haemophilus ducreyi reveals that CDT consists of an enzyme of the DNase-I family, bound to two ricin-like lectin domains. CdtA, CdtB and CdtC form a ternary complex with three interdependent molecular interfaces, characterized by globular, as well as extensive non-globular, interactions. The lectin subunits form a deeply grooved, highly aromatic surface that we show to be critical for toxicity. The holotoxin possesses a steric block of the CdtB active site by means of a non-globular extension of the CdtC subunit, and we identify putative DNA binding residues in CdtB that are essential for toxin activity.
About this Structure
1SR4 is a Protein complex structure of sequences from Haemophilus ducreyi. Full crystallographic information is available from OCA.
Reference
Assembly and function of a bacterial genotoxin., Nesic D, Hsu Y, Stebbins CE, Nature. 2004 May 27;429(6990):429-33. PMID:15164065
Page seeded by OCA on Thu Mar 20 14:08:40 2008
Categories: Haemophilus ducreyi | Protein complex | Hsu, Y. | Nesic, D. | Stebbins, C E. | BR | Apoptosis | Bacterial | Cell cycle | Cytotoxin | Deoxyribonuclease i | Dna damage | Genotoxin | Lectin | Toxin | Virulence
