1ss6
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1ss6.gif|left|200px]] | + | [[Image:1ss6.gif|left|200px]] |
| - | + | ||
| - | '''Solution structure of SEP domain from human p47''' | + | {{Structure |
| + | |PDB= 1ss6 |SIZE=350|CAPTION= <scene name='initialview01'>1ss6</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Solution structure of SEP domain from human p47''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1SS6 is a [ | + | 1SS6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SS6 OCA]. |
==Reference== | ==Reference== | ||
| - | The SEP domain of p47 acts as a reversible competitive inhibitor of cathepsin L., Soukenik M, Diehl A, Leidert M, Sievert V, Bussow K, Leitner D, Labudde D, Ball LJ, Lechner A, Nagler DK, Oschkinat H, FEBS Lett. 2004 Oct 22;576(3):358-62. PMID:[http:// | + | The SEP domain of p47 acts as a reversible competitive inhibitor of cathepsin L., Soukenik M, Diehl A, Leidert M, Sievert V, Bussow K, Leitner D, Labudde D, Ball LJ, Lechner A, Nagler DK, Oschkinat H, FEBS Lett. 2004 Oct 22;576(3):358-62. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15498563 15498563] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 23: | Line 32: | ||
[[Category: nmr; p47; sep]] | [[Category: nmr; p47; sep]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:08:57 2008'' |
Revision as of 12:08, 20 March 2008
| |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution structure of SEP domain from human p47
Overview
The solution structure of the human p47 SEP domain in a construct comprising residues G1-S2-p47(171-270) was determined by NMR spectroscopy. A structure-derived hypothesis about the domains' function was formulated and pursued in binding experiments with cysteine proteases. The SEP domain was found to be a reversible competitive inhibitor of cathepsin L with a Ki of 1.5 microM. The binding of G1-S2-p47(171-270) to cathepsin L was mapped by biochemical assays and the binding interface was investigated by NMR chemical shift perturbation experiments.
About this Structure
1SS6 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The SEP domain of p47 acts as a reversible competitive inhibitor of cathepsin L., Soukenik M, Diehl A, Leidert M, Sievert V, Bussow K, Leitner D, Labudde D, Ball LJ, Lechner A, Nagler DK, Oschkinat H, FEBS Lett. 2004 Oct 22;576(3):358-62. PMID:15498563
Page seeded by OCA on Thu Mar 20 14:08:57 2008
