This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1t0c
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1t0c.gif|left|200px]] | + | [[Image:1t0c.gif|left|200px]] |
| - | + | ||
| - | '''Solution Structure of Human Proinsulin C-Peptide''' | + | {{Structure |
| + | |PDB= 1t0c |SIZE=350|CAPTION= <scene name='initialview01'>1t0c</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= INS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''Solution Structure of Human Proinsulin C-Peptide''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 10: | Line 19: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1T0C is a [ | + | 1T0C is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T0C OCA]. |
==Reference== | ==Reference== | ||
| - | Solution structure of human proinsulin C-peptide., Munte CE, Vilela L, Kalbitzer HR, Garratt RC, FEBS J. 2005 Aug;272(16):4284-93. PMID:[http:// | + | Solution structure of human proinsulin C-peptide., Munte CE, Vilela L, Kalbitzer HR, Garratt RC, FEBS J. 2005 Aug;272(16):4284-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16098208 16098208] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 24: | Line 33: | ||
[[Category: type iii' beta-turn]] | [[Category: type iii' beta-turn]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:12:02 2008'' |
Revision as of 12:12, 20 March 2008
| |||||||
| Gene: | INS (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution Structure of Human Proinsulin C-Peptide
Contents |
Overview
The C-peptide of proinsulin is important for the biosynthesis of insulin, but has been considered for a long time to be biologically inert. Recent studies in diabetic patients have stimulated a new debate about its possible regulatory role, suggesting that it is a hormonally active peptide. We describe structural studies of the C-peptide using 2D NMR spectroscopy. In aqueous solution, the NOE patterns and chemical shifts indicate that the ensemble is a nonrandom structure and contains substructures with defined local conformations. These are more clearly visible in 50% H2O/50% 2,2,2-trifluoroethanol. The N-terminal region (residues 2-5) forms a type I beta-turn, whereas the C-terminal region (residues 27-31) presents the most well-defined structure of the whole molecule including a type III'beta-turn. The C-terminal pentapeptide (EGSLQ) has been suggested to be responsible for chiral interactions with an as yet uncharacterized, probably a G-protein-coupled, receptor. The three central regions of the molecule (residues 9-12, 15-18 and 22-25) show tendencies to form beta-bends. We propose that the structure described here for the C-terminal pentapeptide is consistent with the previously postulated CA knuckle, believed to represent the active site of the C-peptide of human proinsulin.
Disease
Known diseases associated with this structure: Diabetes mellitus, rare form OMIM:[176730], Hyperproinsulinemia, familial OMIM:[176730], MODY, one form OMIM:[176730]
About this Structure
1T0C is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Solution structure of human proinsulin C-peptide., Munte CE, Vilela L, Kalbitzer HR, Garratt RC, FEBS J. 2005 Aug;272(16):4284-93. PMID:16098208
Page seeded by OCA on Thu Mar 20 14:12:02 2008
