1t3q

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[[Image:1t3q.gif|left|200px]]<br /><applet load="1t3q" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1t3q.gif|left|200px]]
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caption="1t3q, resolution 1.80&Aring;" />
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'''Crystal structure of quinoline 2-Oxidoreductase from Pseudomonas Putida 86'''<br />
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{{Structure
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|PDB= 1t3q |SIZE=350|CAPTION= <scene name='initialview01'>1t3q</scene>, resolution 1.80&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MCN:PTERIN+CYTOSINE+DINUCLEOTIDE'>MCN</scene>, <scene name='pdbligand=SMO:DIOXOSULFIDOMOLYBDENUM(VI)+ION'>SMO</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Quinoline_2-oxidoreductase Quinoline 2-oxidoreductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.99.17 1.3.99.17]
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|GENE=
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}}
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'''Crystal structure of quinoline 2-Oxidoreductase from Pseudomonas Putida 86'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1T3Q is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=FES:'>FES</scene>, <scene name='pdbligand=FAD:'>FAD</scene>, <scene name='pdbligand=MCN:'>MCN</scene>, <scene name='pdbligand=SMO:'>SMO</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Quinoline_2-oxidoreductase Quinoline 2-oxidoreductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.99.17 1.3.99.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T3Q OCA].
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1T3Q is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T3Q OCA].
==Reference==
==Reference==
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Active site geometry and substrate recognition of the molybdenum hydroxylase quinoline 2-oxidoreductase., Bonin I, Martins BM, Purvanov V, Fetzner S, Huber R, Dobbek H, Structure. 2004 Aug;12(8):1425-35. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15296736 15296736]
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Active site geometry and substrate recognition of the molybdenum hydroxylase quinoline 2-oxidoreductase., Bonin I, Martins BM, Purvanov V, Fetzner S, Huber R, Dobbek H, Structure. 2004 Aug;12(8):1425-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15296736 15296736]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Pseudomonas putida]]
[[Category: Pseudomonas putida]]
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[[Category: qor]]
[[Category: qor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:09:34 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:13:21 2008''

Revision as of 12:13, 20 March 2008

Image:1t3q.gif


PDB ID 1t3q

Drag the structure with the mouse to rotate
, resolution 1.80Å
Ligands: , , , , and
Activity: Quinoline 2-oxidoreductase, with EC number 1.3.99.17
Coordinates: save as pdb, mmCIF, xml



Crystal structure of quinoline 2-Oxidoreductase from Pseudomonas Putida 86


Overview

The soil bacterium Pseudomonas putida 86 uses quinoline as a sole source of carbon and energy. Quinoline 2-oxidoreductase (Qor) catalyzes the first metabolic step converting quinoline to 2-oxo-1,2-dihydroquinoline. Qor is a member of the molybdenum hydroxylases. The molybdenum ion is coordinated by two ene-dithiolate sulfur atoms, two oxo-ligands, and a catalytically crucial sulfido-ligand, whose position in the active site was controversial. The 1.8 A resolution crystal structure of Qor indicates that the sulfido-ligand occupies the equatorial position at the molybdenum ion. The structural comparison of Qor with the allopurinol-inhibited xanthine dehydrogenase from Rhodobacter capsulatus allows direct insight into the mechanism of substrate recognition and the identification of putative catalytic residues. The active site protein variants QorE743V and QorE743D were analyzed to assess the catalytic role of E743.

About this Structure

1T3Q is a Protein complex structure of sequences from Pseudomonas putida. Full crystallographic information is available from OCA.

Reference

Active site geometry and substrate recognition of the molybdenum hydroxylase quinoline 2-oxidoreductase., Bonin I, Martins BM, Purvanov V, Fetzner S, Huber R, Dobbek H, Structure. 2004 Aug;12(8):1425-35. PMID:15296736

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