1t5k
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1t5k.gif|left|200px]] | + | [[Image:1t5k.gif|left|200px]] |
| - | + | ||
| - | '''Crystal structure of amicyanin substituted with cobalt''' | + | {{Structure |
| + | |PDB= 1t5k |SIZE=350|CAPTION= <scene name='initialview01'>1t5k</scene>, resolution 1.40Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene> and <scene name='pdbligand=PO4:PHOSPHATE ION'>PO4</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= MAUC, AMI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=266 Paracoccus denitrificans]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of amicyanin substituted with cobalt''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1T5K is a [ | + | 1T5K is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Paracoccus_denitrificans Paracoccus denitrificans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T5K OCA]. |
==Reference== | ==Reference== | ||
| - | Crystallographic and NMR investigation of cobalt-substituted amicyanin., Carrell CJ, Wang X, Jones L, Jarrett WL, Davidson VL, Mathews FS, Biochemistry. 2004 Jul 27;43(29):9381-9. PMID:[http:// | + | Crystallographic and NMR investigation of cobalt-substituted amicyanin., Carrell CJ, Wang X, Jones L, Jarrett WL, Davidson VL, Mathews FS, Biochemistry. 2004 Jul 27;43(29):9381-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15260481 15260481] |
[[Category: Paracoccus denitrificans]] | [[Category: Paracoccus denitrificans]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 23: | Line 32: | ||
[[Category: electron transport]] | [[Category: electron transport]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:14:04 2008'' |
Revision as of 12:14, 20 March 2008
| |||||||
| , resolution 1.40Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | MAUC, AMI (Paracoccus denitrificans) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of amicyanin substituted with cobalt
Overview
Cobalt(II) amicyanin was prepared by replacing the copper of the type I copper protein amicyanin from Paracoccus denitrificans with cobalt. The structure of the protein and the metal center have been characterized by X-ray crystallography and paramagnetic NMR spectroscopy. The crystal structure indicates that Met98, which provides an axial sulfur ligand in native amicyanin, is no longer bound to the metal in cobalt(II) amicyanin and that a water molecule is recruited from solvent to form the fourth metal ligand. This results in a tetrahedral coordination geometry for the cobalt ion. NMR studies in solution also indicate that the side chain of the methionine residue interacts less strongly with the metal in P. denitrificans amicyanin than in Paracoccus versutus amicyanin. The cobalt(II) amicyanin crystal structure is different from that of cobalt-substituted azurin in which the carbonyl of a glycine residue provides this equivalent ligand. In cobalt(II) amicyanin that residue is a proline, for which the oxygen is structurally inaccessible, so that the water occupies the position held by the glycine carbonyl in cobalt(II) azurin. Such a metal coordination involving water has not previously been reported for a native or metal-substituted type I copper protein.
About this Structure
1T5K is a Single protein structure of sequence from Paracoccus denitrificans. Full crystallographic information is available from OCA.
Reference
Crystallographic and NMR investigation of cobalt-substituted amicyanin., Carrell CJ, Wang X, Jones L, Jarrett WL, Davidson VL, Mathews FS, Biochemistry. 2004 Jul 27;43(29):9381-9. PMID:15260481
Page seeded by OCA on Thu Mar 20 14:14:04 2008
