1t8z
From Proteopedia
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| - | [[Image:1t8z.jpg|left|200px]] | + | [[Image:1t8z.jpg|left|200px]] |
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| - | '''Atomic Structure of A Novel Tryptophan-Zipper Pentamer''' | + | {{Structure |
| + | |PDB= 1t8z |SIZE=350|CAPTION= <scene name='initialview01'>1t8z</scene>, resolution 1.45Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=12P:DODECAETHYLENE GLYCOL'>12P</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= LPP, MLPA, MULI, B1677, C2072, Z2705, ECS2384, SF1706, S1839 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | }} | ||
| + | |||
| + | '''Atomic Structure of A Novel Tryptophan-Zipper Pentamer''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1T8Z is a [ | + | 1T8Z is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8Z OCA]. |
==Reference== | ==Reference== | ||
| - | Atomic structure of a tryptophan-zipper pentamer., Liu J, Yong W, Deng Y, Kallenbach NR, Lu M, Proc Natl Acad Sci U S A. 2004 Nov 16;101(46):16156-61. Epub 2004 Nov 1. PMID:[http:// | + | Atomic structure of a tryptophan-zipper pentamer., Liu J, Yong W, Deng Y, Kallenbach NR, Lu M, Proc Natl Acad Sci U S A. 2004 Nov 16;101(46):16156-61. Epub 2004 Nov 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15520380 15520380] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: tryptophan-zipper]] | [[Category: tryptophan-zipper]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:15:25 2008'' |
Revision as of 12:15, 20 March 2008
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| , resolution 1.45Å | |||||||
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| Ligands: | and | ||||||
| Gene: | LPP, MLPA, MULI, B1677, C2072, Z2705, ECS2384, SF1706, S1839 (Escherichia coli) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Atomic Structure of A Novel Tryptophan-Zipper Pentamer
Overview
Coiled-coil motifs are ubiquitous mediators of specific protein-protein interactions through the formation of interlocking hydrophobic seams between alpha-helical chains. Residues that form these seams occur at the first (a) and fourth (d) positions of a characteristic 7-aa repeat and are primarily aliphatic. The potential of aromatic residues to promote helix association in a coiled coil was explored by engineering a "Trp-zipper" protein with Trp residues at all 14 a and d positions. The protein forms a discrete, stable, alpha-helical pentamer in water at physiological pH. Its 1.45-A crystal structure reveals a parallel, five-stranded coiled coil, a previously uncharacterized type of "knobs-into-holes" packing interaction between interfacial Trp side chains, and an unusual approximately 8-A-diameter axial channel lined with indole rings that is filled with polyethylene glycol 400 and water and sulfate ion molecules. The engineered Trp-zipper pentamer enlarges current views of coiled-coil assembly, molecular recognition, and protein engineering, and may serve as a soluble model for membrane ion channels.
About this Structure
1T8Z is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Atomic structure of a tryptophan-zipper pentamer., Liu J, Yong W, Deng Y, Kallenbach NR, Lu M, Proc Natl Acad Sci U S A. 2004 Nov 16;101(46):16156-61. Epub 2004 Nov 1. PMID:15520380
Page seeded by OCA on Thu Mar 20 14:15:25 2008
Categories: Escherichia coli | Single protein | Deng, Y. | Kallenbach, N R. | Liu, J. | Lu, M. | Yong, W. | 12P | SO4 | Coiled coil | Lipoprotein | Pentamer | Protein folding | Tryptophan-zipper
