1tc8

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[[Image:1tc8.jpg|left|200px]]<br /><applet load="1tc8" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1tc8.jpg|left|200px]]
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caption="1tc8, resolution 2.70&Aring;" />
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'''Crystal structure of Krait-venom phospholipase A2 in a complex with a natural fatty acid tridecanoic acid'''<br />
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{{Structure
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|PDB= 1tc8 |SIZE=350|CAPTION= <scene name='initialview01'>1tc8</scene>, resolution 2.70&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> and <scene name='pdbligand=TDA:N-TRIDECANOIC ACID'>TDA</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4]
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|GENE=
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}}
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'''Crystal structure of Krait-venom phospholipase A2 in a complex with a natural fatty acid tridecanoic acid'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1TC8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bungarus_caeruleus Bungarus caeruleus] with <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=TDA:'>TDA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TC8 OCA].
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1TC8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bungarus_caeruleus Bungarus caeruleus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TC8 OCA].
==Reference==
==Reference==
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Crystal structure of the complex formed between a group I phospholipase A2 and a naturally occurring fatty acid at 2.7 A resolution., Singh G, Jasti J, Saravanan K, Sharma S, Kaur P, Srinivasan A, Singh TP, Protein Sci. 2005 Feb;14(2):395-400. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15659372 15659372]
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Crystal structure of the complex formed between a group I phospholipase A2 and a naturally occurring fatty acid at 2.7 A resolution., Singh G, Jasti J, Saravanan K, Sharma S, Kaur P, Srinivasan A, Singh TP, Protein Sci. 2005 Feb;14(2):395-400. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15659372 15659372]
[[Category: Bungarus caeruleus]]
[[Category: Bungarus caeruleus]]
[[Category: Phospholipase A(2)]]
[[Category: Phospholipase A(2)]]
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[[Category: phospholipasea2]]
[[Category: phospholipasea2]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:12:03 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:16:33 2008''

Revision as of 12:16, 20 March 2008

Image:1tc8.jpg


PDB ID 1tc8

Drag the structure with the mouse to rotate
, resolution 2.70Å
Ligands: and
Activity: Phospholipase A(2), with EC number 3.1.1.4
Coordinates: save as pdb, mmCIF, xml



Crystal structure of Krait-venom phospholipase A2 in a complex with a natural fatty acid tridecanoic acid


Overview

This is the first evidence of a naturally bound fatty acid to a group I Phospholipase A(2) (PLA(2)) and also to a PLA(2) with Asp 49. The fatty acid identified as n-tridecanoic acid is observed at the substrate recognition site of PLA(2) hydrophobic channel. The complex was isolated from the venom of Bungarus caeruleus (Common Indian Krait). The primary sequence of the PLA(2) was determined using the cDNA method. Three-dimensional structure has been solved by the molecular replacement method and refined using the CNS package to a final R factor of 19.8% for the data in the resolution range from 20.0 to 2.7 A. The final refined model is comprised of 912 protein atoms, one sodium ion, one molecule of n-tridecanoic acid, and 60 water molecules. The sodium ion is located in the calcium-binding loop with a sevenfold coordination. A characteristic extra electron density was observed in the hydrophobic channel of the enzyme, into which a molecule of n-tridecanoic acid was clearly fitted. The MALDI-TOF measurements of the crystals had earlier indicated an increase in the molecular mass of PLA(2) by 212 Da over the native PLA(2). A major part of the ligand fits well in the binding pocket and interacts directly with His 48 and Asp 49. Although the overall structure of PLA(2) in the present complex is similar to the native structure reported earlier, it differs significantly in the folding of its calcium-binding loop.

About this Structure

1TC8 is a Single protein structure of sequence from Bungarus caeruleus. Full crystallographic information is available from OCA.

Reference

Crystal structure of the complex formed between a group I phospholipase A2 and a naturally occurring fatty acid at 2.7 A resolution., Singh G, Jasti J, Saravanan K, Sharma S, Kaur P, Srinivasan A, Singh TP, Protein Sci. 2005 Feb;14(2):395-400. PMID:15659372

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