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From Proteopedia
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===Glycoside Hydrolysases=== | ===Glycoside Hydrolysases=== | ||
| - | Glycoside hydrolases (GH) constitute a widespread enzyme group presenting a huge variety of protein folds and substrate specificities. They are classified into EC 3.2.1 as enzymes catalyzing the hydrolysis of O-, N- and S-linked glycosides. The enzymatic hydrolysis of glycosidic bonds can be realised by two ways : inversion or retention of the product anomeric configuration compared with the substrate anomeric configuration. So we can classify Glycoside hydrolases according to these mechanismes. But we can alson make a classification depending on three-dimensional structure or on sequence. The classification based on sequence allowed to define more than 120 families of Glycoside hydrolases : GH9, GH14... The ''Thermotoga maritima'' β-fructosidase belongs to the GH32 family. | + | Glycoside hydrolases (GH)<ref>Glycoside Hydrolase family classification, [http://www.cazy.org/Glycoside-Hydrolases.html]<ref> constitute a widespread enzyme group presenting a huge variety of protein folds and substrate specificities. They are classified into EC 3.2.1 as enzymes catalyzing the hydrolysis of O-, N- and S-linked glycosides. The enzymatic hydrolysis of glycosidic bonds can be realised by two ways : inversion or retention of the product anomeric configuration compared with the substrate anomeric configuration. So we can classify Glycoside hydrolases according to these mechanismes. But we can alson make a classification depending on three-dimensional structure or on sequence. The classification based on sequence allowed to define more than 120 families of Glycoside hydrolases : GH9, GH14... The ''Thermotoga maritima'' β-fructosidase belongs to the GH32 family. |
===GH32 Family=== | ===GH32 Family=== | ||
Revision as of 17:46, 2 January 2013
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Thermotoga maritima β-fructosidase (invertase)
Introduction
The β-fructosidase (β-D-fructofuranosidase EC 3. 2. 1. 26.) of the thermophilic baterium Thermotoga maritima is an enzyme which hydrolyzes sucrose to release an equimolar mixture of fructose and glucose, called inberted sugar. That is why the other name of the β-fructosidase is invertase. 
This enzyme achieves the hydrolysis with a retention molecular mechanism. Actually, the invertase hydrolyses the D-sucrose to form the D-fructose and the D-glucose. So, the substrate anomeric configuration is conserved in products. The β-fructosidase belongs to the GH32 (Glycoside hydrolysases 32) according to the sequence-based classification of glycoside hydrolysases. The β-fructosidase hydrolyses other substrates like raffinose or stachyose but here we will focus on sucrose hysrolyse.
Glycoside Hydrolases and GH32 family
Glycoside Hydrolysases
Glycoside hydrolases (GH)[1]
