1tez
From Proteopedia
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| - | [[Image:1tez.gif|left|200px]] | + | [[Image:1tez.gif|left|200px]] |
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| - | '''COMPLEX BETWEEN DNA AND THE DNA PHOTOLYASE FROM ANACYSTIS NIDULANS''' | + | {{Structure |
| + | |PDB= 1tez |SIZE=350|CAPTION= <scene name='initialview01'>1tez</scene>, resolution 1.80Å | ||
| + | |SITE= <scene name='pdbsite=ACT:Upon+Complexation+w.+The+Cpd-Damage+The+Active+Site+Appa+...'>ACT</scene> | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene> and <scene name='pdbligand=HDF:8-HYDROXY-10-(D-RIBO-2,3,4,5-TETRAHYDROXYPENTYL)-5-DEAZAISOALLOXAZINE'>HDF</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Deoxyribodipyrimidine_photo-lyase Deoxyribodipyrimidine photo-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.99.3 4.1.99.3] | ||
| + | |GENE= PHR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1131 Synechococcus sp.]) | ||
| + | }} | ||
| + | |||
| + | '''COMPLEX BETWEEN DNA AND THE DNA PHOTOLYASE FROM ANACYSTIS NIDULANS''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1TEZ is a [ | + | 1TEZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Synechococcus_sp. Synechococcus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TEZ OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of a photolyase bound to a CPD-like DNA lesion after in situ repair., Mees A, Klar T, Gnau P, Hennecke U, Eker AP, Carell T, Essen LO, Science. 2004 Dec 3;306(5702):1789-93. PMID:[http:// | + | Crystal structure of a photolyase bound to a CPD-like DNA lesion after in situ repair., Mees A, Klar T, Gnau P, Hennecke U, Eker AP, Carell T, Essen LO, Science. 2004 Dec 3;306(5702):1789-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15576622 15576622] |
[[Category: Deoxyribodipyrimidine photo-lyase]] | [[Category: Deoxyribodipyrimidine photo-lyase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: photolyase; dna repair; light-driven electron transfer]] | [[Category: photolyase; dna repair; light-driven electron transfer]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:17:28 2008'' |
Revision as of 12:17, 20 March 2008
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| , resolution 1.80Å | |||||||
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| Sites: | |||||||
| Ligands: | , and | ||||||
| Gene: | PHR (Synechococcus sp.) | ||||||
| Activity: | Deoxyribodipyrimidine photo-lyase, with EC number 4.1.99.3 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
COMPLEX BETWEEN DNA AND THE DNA PHOTOLYASE FROM ANACYSTIS NIDULANS
Overview
DNA photolyases use light energy to repair DNA that comprises ultraviolet-induced lesions such as the cis-syn cyclobutane pyrimidine dimers (CPDs). Here we report the crystal structure of a DNA photolyase bound to duplex DNA that is bent by 50 degrees and comprises a synthetic CPD lesion. This CPD lesion is flipped into the active site and split there into two thymines by synchrotron radiation at 100 K. Although photolyases catalyze blue light-driven CPD cleavage only above 200 K, this structure apparently mimics a structural substate during light-driven DNA repair in which back-flipping of the thymines into duplex DNA has not yet taken place.
About this Structure
1TEZ is a Single protein structure of sequence from Synechococcus sp.. Full crystallographic information is available from OCA.
Reference
Crystal structure of a photolyase bound to a CPD-like DNA lesion after in situ repair., Mees A, Klar T, Gnau P, Hennecke U, Eker AP, Carell T, Essen LO, Science. 2004 Dec 3;306(5702):1789-93. PMID:15576622
Page seeded by OCA on Thu Mar 20 14:17:28 2008
