1tg7

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[[Image:1tg7.gif|left|200px]]<br /><applet load="1tg7" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1tg7.gif|left|200px]]
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caption="1tg7, resolution 1.90&Aring;" />
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'''Native structure of beta-galactosidase from Penicillium sp.'''<br />
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{{Structure
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|PDB= 1tg7 |SIZE=350|CAPTION= <scene name='initialview01'>1tg7</scene>, resolution 1.90&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> and <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Beta-galactosidase Beta-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.23 3.2.1.23]
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|GENE=
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}}
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'''Native structure of beta-galactosidase from Penicillium sp.'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1TG7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Penicillium_soppii Penicillium soppii] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Beta-galactosidase Beta-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.23 3.2.1.23] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TG7 OCA].
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1TG7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Penicillium_soppii Penicillium soppii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TG7 OCA].
==Reference==
==Reference==
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Crystal structures of beta-galactosidase from Penicillium sp. and its complex with galactose., Rojas AL, Nagem RA, Neustroev KN, Arand M, Adamska M, Eneyskaya EV, Kulminskaya AA, Garratt RC, Golubev AM, Polikarpov I, J Mol Biol. 2004 Nov 5;343(5):1281-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15491613 15491613]
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Crystal structures of beta-galactosidase from Penicillium sp. and its complex with galactose., Rojas AL, Nagem RA, Neustroev KN, Arand M, Adamska M, Eneyskaya EV, Kulminskaya AA, Garratt RC, Golubev AM, Polikarpov I, J Mol Biol. 2004 Nov 5;343(5):1281-92. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15491613 15491613]
[[Category: Beta-galactosidase]]
[[Category: Beta-galactosidase]]
[[Category: Penicillium soppii]]
[[Category: Penicillium soppii]]
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[[Category: tim barrel domain]]
[[Category: tim barrel domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:13:11 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:17:59 2008''

Revision as of 12:18, 20 March 2008

Image:1tg7.gif


PDB ID 1tg7

Drag the structure with the mouse to rotate
, resolution 1.90Å
Ligands: , , and
Activity: Beta-galactosidase, with EC number 3.2.1.23
Coordinates: save as pdb, mmCIF, xml



Native structure of beta-galactosidase from Penicillium sp.


Overview

Beta-galactosidases catalyze the hydrolysis of beta(1-3) and beta(1-4) galactosyl bonds in oligosaccharides as well as the inverse reaction of enzymatic condensation and transglycosylation. Here we report the crystallographic structures of Penicillium sp. beta-galactosidase and its complex with galactose solved by the SIRAS quick cryo-soaking technique at 1.90 A and 2.10 A resolution, respectively. The amino acid sequence of this 120 kDa protein was first assigned putatively on the basis of inspection of the experimental electron density maps and then determined by nucleotide sequence analysis. Primary structure alignments reveal that Penicillium sp. beta-galactosidase belongs to family 35 of glycosyl hydrolases (GHF-35). This model is the first 3D structure for a member of GHF-35. Five distinct domains which comprise the structure are assembled in a way previously unobserved for beta-galactosidases. Superposition of this complex with other beta-galactosidase complexes from several hydrolase families allowed the identification of residue Glu200 as the proton donor and residue Glu299 as the nucleophile involved in catalysis. Penicillium sp. beta-galactosidase is a glycoprotein containing seven N-linked oligosaccharide chains and is the only structure of a glycosylated beta-galactosidase described to date.

About this Structure

1TG7 is a Single protein structure of sequence from Penicillium soppii. Full crystallographic information is available from OCA.

Reference

Crystal structures of beta-galactosidase from Penicillium sp. and its complex with galactose., Rojas AL, Nagem RA, Neustroev KN, Arand M, Adamska M, Eneyskaya EV, Kulminskaya AA, Garratt RC, Golubev AM, Polikarpov I, J Mol Biol. 2004 Nov 5;343(5):1281-92. PMID:15491613

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