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1tgl
From Proteopedia
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| - | [[Image:1tgl.jpg|left|200px]] | + | [[Image:1tgl.jpg|left|200px]] |
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| - | '''A SERINE PROTEASE TRIAD FORMS THE CATALYTIC CENTRE OF A TRIACYLGLYCEROL LIPASE''' | + | {{Structure |
| + | |PDB= 1tgl |SIZE=350|CAPTION= <scene name='initialview01'>1tgl</scene>, resolution 1.9Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''A SERINE PROTEASE TRIAD FORMS THE CATALYTIC CENTRE OF A TRIACYLGLYCEROL LIPASE''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1TGL is a [ | + | 1TGL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rhizomucor_miehei Rhizomucor miehei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TGL OCA]. |
==Reference== | ==Reference== | ||
| - | A serine protease triad forms the catalytic centre of a triacylglycerol lipase., Brady L, Brzozowski AM, Derewenda ZS, Dodson E, Dodson G, Tolley S, Turkenburg JP, Christiansen L, Huge-Jensen B, Norskov L, et al., Nature. 1990 Feb 22;343(6260):767-70. PMID:[http:// | + | A serine protease triad forms the catalytic centre of a triacylglycerol lipase., Brady L, Brzozowski AM, Derewenda ZS, Dodson E, Dodson G, Tolley S, Turkenburg JP, Christiansen L, Huge-Jensen B, Norskov L, et al., Nature. 1990 Feb 22;343(6260):767-70. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/2304552 2304552] |
[[Category: Rhizomucor miehei]] | [[Category: Rhizomucor miehei]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: hydrolase(carboxylic esterase)]] | [[Category: hydrolase(carboxylic esterase)]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:18:09 2008'' |
Revision as of 12:18, 20 March 2008
| |||||||
| , resolution 1.9Å | |||||||
|---|---|---|---|---|---|---|---|
| Activity: | Triacylglycerol lipase, with EC number 3.1.1.3 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
A SERINE PROTEASE TRIAD FORMS THE CATALYTIC CENTRE OF A TRIACYLGLYCEROL LIPASE
Overview
True lipases attach triacylglycerols and act at an oil-water interface; they constitute a ubiquitous group of enzymes catalysing a wide variety of reactions, many with industrial potential. But so far the three-dimensional structure has not been reported for any lipase. Here we report the X-ray structure of the Mucor miehei triglyceride lipase and describe the atomic model obtained at 3.1 A resolution and refined to 1.9 A resolution. It reveals a Ser..His..Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.
About this Structure
1TGL is a Single protein structure of sequence from Rhizomucor miehei. Full crystallographic information is available from OCA.
Reference
A serine protease triad forms the catalytic centre of a triacylglycerol lipase., Brady L, Brzozowski AM, Derewenda ZS, Dodson E, Dodson G, Tolley S, Turkenburg JP, Christiansen L, Huge-Jensen B, Norskov L, et al., Nature. 1990 Feb 22;343(6260):767-70. PMID:2304552
Page seeded by OCA on Thu Mar 20 14:18:09 2008
