1rwd

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[[Image:1rwd.png|left|200px]]
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==Backbone NMR Structure of a Mutant P. Furiosus Rubredoxin Using Residual Dipolar Couplings==
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<StructureSection load='1rwd' size='340' side='right' caption='[[1rwd]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1rwd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1m2y 1m2y]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RWD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RWD FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1m2y|1m2y]]</td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RUB, PF1282 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2261 Pyrococcus furiosus])</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rwd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rwd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1rwd RCSB], [http://www.ebi.ac.uk/pdbsum/1rwd PDBsum], [http://www.topsan.org/Proteins/SECSG/1rwd TOPSAN]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rw/1rwd_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A new approach for simultaneous protein backbone resonance assignment and structure determination by NMR is introduced. This approach relies on recent advances in high-resolution NMR spectroscopy that allow observation of anisotropic interactions, such as dipolar couplings, from proteins partially aligned in field ordered media. Residual dipolar couplings are used for both geometric information and a filter in the assembly of residues in a sequential manner. Experimental data were collected in less than one week on a small redox protein, rubredoxin, that was 15N enriched but not enriched above 1% natural abundance in 13C. Given the acceleration possible with partial 13C enrichment, the protocol described should provide a very rapid route to protein structure determination. This is critical for the structural genomics initiative where protein expression and structural determination in a high-throughput manner will be needed.
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{{STRUCTURE_1rwd| PDB=1rwd | SCENE= }}
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A dipolar coupling based strategy for simultaneous resonance assignment and structure determination of protein backbones.,Tian F, Valafar H, Prestegard JH J Am Chem Soc. 2001 Nov 28;123(47):11791-6. PMID:11716736<ref>PMID:11716736</ref>
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===Backbone NMR Structure of a Mutant P. Furiosus Rubredoxin Using Residual Dipolar Couplings===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_11716736}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[1rwd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1m2y 1m2y]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RWD OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:011716736</ref><references group="xtra"/>
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[[Category: Pyrococcus furiosus]]
[[Category: Pyrococcus furiosus]]
[[Category: Prestegard, J H.]]
[[Category: Prestegard, J H.]]

Revision as of 14:27, 29 September 2014

Backbone NMR Structure of a Mutant P. Furiosus Rubredoxin Using Residual Dipolar Couplings

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