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1s9h
From Proteopedia
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| - | [[ | + | ==Crystal Structure of Adeno-associated virus Type 2 Rep40== |
| + | <StructureSection load='1s9h' size='340' side='right' caption='[[1s9h]], [[Resolution|resolution]] 2.40Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1s9h]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Adeno-associated_virus_-_2 Adeno-associated virus - 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S9H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1S9H FirstGlance]. <br> | ||
| + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">REP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10804 Adeno-associated virus - 2])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s9h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1s9h RCSB], [http://www.ebi.ac.uk/pdbsum/1s9h PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s9/1s9h_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | We report here the crystal structure of an SF3 DNA helicase, Rep40, from adeno-associated virus 2 (AAV2). We show that AAV2 Rep40 is structurally more similar to the AAA(+) class of cellular proteins than to DNA helicases from other superfamilies. The structure delineates the expected Walker A and B motifs, but also reveals an unexpected "arginine finger" that directly implies the requirement of Rep40 oligomerization for ATP hydrolysis and helicase activity. Further, the Rep40 AAA(+) domain is novel in that it is unimodular as opposed to bimodular. Altogether, the structural connection to AAA(+) proteins defines the general architecture of SF3 DNA helicases, a family that includes simian virus 40 (SV40) T antigen, as well as provides a conceptual framework for understanding the role of Rep proteins during AAV DNA replication, packaging, and site-specific integration. | ||
| - | + | Crystal structure of the SF3 helicase from adeno-associated virus type 2.,James JA, Escalante CR, Yoon-Robarts M, Edwards TA, Linden RM, Aggarwal AK Structure. 2003 Aug;11(8):1025-35. PMID:12906833<ref>PMID:12906833</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | + | </StructureSection> | |
| - | + | ||
| - | == | + | |
| - | < | + | |
[[Category: Adeno-associated virus - 2]] | [[Category: Adeno-associated virus - 2]] | ||
| - | [[Category: Aggarwal, A K | + | [[Category: Aggarwal, A K]] |
| - | [[Category: Edwards, T A | + | [[Category: Edwards, T A]] |
| - | [[Category: Escalante, C R | + | [[Category: Escalante, C R]] |
| - | [[Category: James, J A | + | [[Category: James, J A]] |
| - | [[Category: Linden, R M | + | [[Category: Linden, R M]] |
| - | [[Category: Yoon-Robarts, M | + | [[Category: Yoon-Robarts, M]] |
[[Category: Aaa+ protein]] | [[Category: Aaa+ protein]] | ||
[[Category: Helicase]] | [[Category: Helicase]] | ||
Revision as of 07:59, 6 January 2015
Crystal Structure of Adeno-associated virus Type 2 Rep40
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