1tk7
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1tk7.gif|left|200px]] | + | [[Image:1tk7.gif|left|200px]] |
| - | + | ||
| - | '''NMR structure of WW domains (WW3-4) from Suppressor of Deltex''' | + | {{Structure |
| + | |PDB= 1tk7 |SIZE=350|CAPTION= <scene name='initialview01'>1tk7</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= Su(dx) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster]) | ||
| + | }} | ||
| + | |||
| + | '''NMR structure of WW domains (WW3-4) from Suppressor of Deltex''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1TK7 is a [ | + | 1TK7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TK7 OCA]. |
==Reference== | ==Reference== | ||
| - | The structure and dynamics of tandem WW domains in a negative regulator of notch signaling, Suppressor of deltex., Fedoroff OY, Townson SA, Golovanov AP, Baron M, Avis JM, J Biol Chem. 2004 Aug 13;279(33):34991-5000. Epub 2004 Jun 1. PMID:[http:// | + | The structure and dynamics of tandem WW domains in a negative regulator of notch signaling, Suppressor of deltex., Fedoroff OY, Townson SA, Golovanov AP, Baron M, Avis JM, J Biol Chem. 2004 Aug 13;279(33):34991-5000. Epub 2004 Jun 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15173166 15173166] |
[[Category: Drosophila melanogaster]] | [[Category: Drosophila melanogaster]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 21: | Line 30: | ||
[[Category: ww domain]] | [[Category: ww domain]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:19:28 2008'' |
Revision as of 12:19, 20 March 2008
| |||||||
| Gene: | Su(dx) (Drosophila melanogaster) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NMR structure of WW domains (WW3-4) from Suppressor of Deltex
Overview
WW domains mediate protein recognition, usually though binding to proline-rich sequences. In many proteins, WW domains occur in tandem arrays. Whether or how individual domains within such arrays cooperate to recognize biological partners is, as yet, poorly characterized. An important question is whether functional diversity of different WW domain proteins is reflected in the structural organization and ligand interaction mechanisms of their multiple domains. We have determined the solution structure and dynamics of a pair of WW domains (WW3-4) from a Drosophila Nedd4 family protein called Suppressor of deltex (Su(dx)), a regulator of Notch receptor signaling. We find that the binding of a type 1 PPPY ligand to WW3 stabilizes the structure with effects propagating to the WW4 domain, a domain that is not active for ligand binding. Both WW domains adopt the characteristic triple-stranded beta-sheet structure, and significantly, this is the first example of a WW domain structure to include a domain (WW4) lacking the second conserved Trp (replaced by Phe). The domains are connected by a flexible linker, which allows a hinge-like motion of domains that may be important for the recognition of functionally relevant targets. Our results contrast markedly with those of the only previously determined three-dimensional structure of tandem WW domains, that of the rigidly oriented WW domain pair from the RNA-splicing factor Prp40. Our data illustrate that arrays of WW domains can exhibit a variety of higher order structures and ligand interaction mechanisms.
About this Structure
1TK7 is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
The structure and dynamics of tandem WW domains in a negative regulator of notch signaling, Suppressor of deltex., Fedoroff OY, Townson SA, Golovanov AP, Baron M, Avis JM, J Biol Chem. 2004 Aug 13;279(33):34991-5000. Epub 2004 Jun 1. PMID:15173166
Page seeded by OCA on Thu Mar 20 14:19:28 2008
