1uo9

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[[Image:1uo9.png|left|200px]]
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==DEACETOXYCEPHALOSPORIN C SYNTHASE COMPLEXED WITH SUCCINATE==
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<StructureSection load='1uo9' size='340' side='right' caption='[[1uo9]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1uo9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UO9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UO9 FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=SIN:SUCCINIC+ACID'>SIN</scene><br>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1dcs|1dcs]], [[1e5h|1e5h]], [[1e5i|1e5i]], [[1hjf|1hjf]], [[1hjg|1hjg]], [[1rxf|1rxf]], [[1rxg|1rxg]], [[1unb|1unb]], [[1uob|1uob]], [[1uof|1uof]], [[1uog|1uog]]</td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deacetoxycephalosporin-C_synthase Deacetoxycephalosporin-C synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.20.1 1.14.20.1] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uo9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uo9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1uo9 RCSB], [http://www.ebi.ac.uk/pdbsum/1uo9 PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uo/1uo9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Deacetoxycephalosporin-C synthase (DAOCS) is a mononuclear ferrous enzyme that transforms penicillins into cephalosporins by inserting a carbon atom into the penicillin nucleus. In the first half-reaction, dioxygen and 2-oxoglutarate produce a reactive iron-oxygen species, succinate and CO2. The oxidizing iron species subsequently reacts with penicillin to give cephalosporin and water. Here we describe high-resolution structures for ferrous DAOCS in complex with penicillins, the cephalosporin product, the cosubstrate and the coproduct. Steady-state kinetic data, quantum-chemical calculations and the new structures indicate a reaction sequence in which a 'booby-trapped' oxidizing species is formed. This species is stabilized by the negative charge of succinate on the iron. The binding sites of succinate and penicillin overlap, and when penicillin replaces succinate, it removes the stabilizing charge, eliciting oxidative attack on itself. Requisite groups of penicillin are within 1 A of the expected position of a ferryl oxygen in the enzyme-penicillin complex.
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{{STRUCTURE_1uo9| PDB=1uo9 | SCENE= }}
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The structural basis of cephalosporin formation in a mononuclear ferrous enzyme.,Valegard K, Terwisscha van Scheltinga AC, Dubus A, Ranghino G, Oster LM, Hajdu J, Andersson I Nat Struct Mol Biol. 2004 Jan;11(1):95-101. Epub 2003 Dec 29. PMID:14718929<ref>PMID:14718929</ref>
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===DEACETOXYCEPHALOSPORIN C SYNTHASE COMPLEXED WITH SUCCINATE===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_14718929}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[1uo9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UO9 OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:014718929</ref><ref group="xtra">PMID:014514675</ref><references group="xtra"/>
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[[Category: Deacetoxycephalosporin-C synthase]]
[[Category: Deacetoxycephalosporin-C synthase]]
[[Category: Streptomyces clavuligerus]]
[[Category: Streptomyces clavuligerus]]

Revision as of 18:21, 29 September 2014

DEACETOXYCEPHALOSPORIN C SYNTHASE COMPLEXED WITH SUCCINATE

1uo9, resolution 1.50Å

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