1tto
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1tto.gif|left|200px]] | + | [[Image:1tto.gif|left|200px]] |
| - | + | ||
| - | '''Crystal structure of the Rnase T1 variant R2''' | + | {{Structure |
| + | |PDB= 1tto |SIZE=350|CAPTION= <scene name='initialview01'>1tto</scene>, resolution 2.1Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of the Rnase T1 variant R2''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1TTO is a [ | + | 1TTO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TTO OCA]. |
==Reference== | ==Reference== | ||
| - | Purine activity of RNase T1RV is further improved by substitution of Trp59 by tyrosine., Czaja R, Perbandt M, Betzel C, Hahn U, Biochem Biophys Res Commun. 2005 Oct 28;336(3):882-9. PMID:[http:// | + | Purine activity of RNase T1RV is further improved by substitution of Trp59 by tyrosine., Czaja R, Perbandt M, Betzel C, Hahn U, Biochem Biophys Res Commun. 2005 Oct 28;336(3):882-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16157302 16157302] |
[[Category: Aspergillus oryzae]] | [[Category: Aspergillus oryzae]] | ||
[[Category: Ribonuclease T(1)]] | [[Category: Ribonuclease T(1)]] | ||
| Line 23: | Line 32: | ||
[[Category: ribonuclease]] | [[Category: ribonuclease]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:23:00 2008'' |
Revision as of 12:23, 20 March 2008
| |||||||
| , resolution 2.1Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Activity: | Ribonuclease T(1), with EC number 3.1.27.3 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the Rnase T1 variant R2
Overview
Ribonuclease T1 is an enzyme that cleaves single-stranded RNA with high specificity after guanylyl residues. Although this enzyme is a very good characterized protein with respect to structure and enzymatic function, we were only recently successful in generating RNase T1-RV, a variant where the specificity was changed from guanine to purine. As this change of substrate specificity was made at the cost of activity, the aim was now to further improve the overall activity of the enzyme. Therefore, we have substituted the tryptophan in position 59 by tyrosine. This substitution led to an increase of enzymatic activity in comparison to variant RV to 425%. As the extent of this enhancement is unique so far we have crystallized and analyzed the structure of this variant in order to get more insights into the reasons for this. Here, we present the crystal structure of this so-called RNase T1-R2 at 2.1A resolution. The structure was determined by molecular replacement using the coordinates of the RV variant (PDB entry: 1Q9E). The data were refined to an R-factor of 18.7% and R(free) of 24%, respectively. The asymmetric unit contains three molecules and the crystal packing is very similar to that of variant RV.
About this Structure
1TTO is a Single protein structure of sequence from Aspergillus oryzae. Full crystallographic information is available from OCA.
Reference
Purine activity of RNase T1RV is further improved by substitution of Trp59 by tyrosine., Czaja R, Perbandt M, Betzel C, Hahn U, Biochem Biophys Res Commun. 2005 Oct 28;336(3):882-9. PMID:16157302
Page seeded by OCA on Thu Mar 20 14:23:00 2008
