1txu
From Proteopedia
Line 1: | Line 1: | ||
- | [[Image:1txu.jpg|left|200px]] | + | [[Image:1txu.jpg|left|200px]] |
- | + | ||
- | '''Crystal Structure of the Vps9 Domain of Rabex-5''' | + | {{Structure |
+ | |PDB= 1txu |SIZE=350|CAPTION= <scene name='initialview01'>1txu</scene>, resolution 2.35Å | ||
+ | |SITE= | ||
+ | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | ||
+ | |ACTIVITY= | ||
+ | |GENE= RABGEF1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
+ | }} | ||
+ | |||
+ | '''Crystal Structure of the Vps9 Domain of Rabex-5''' | ||
+ | |||
==Overview== | ==Overview== | ||
Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
- | 1TXU is a [ | + | 1TXU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TXU OCA]. |
==Reference== | ==Reference== | ||
- | Structure, exchange determinants, and family-wide rab specificity of the tandem helical bundle and Vps9 domains of Rabex-5., Delprato A, Merithew E, Lambright DG, Cell. 2004 Sep 3;118(5):607-17. PMID:[http:// | + | Structure, exchange determinants, and family-wide rab specificity of the tandem helical bundle and Vps9 domains of Rabex-5., Delprato A, Merithew E, Lambright DG, Cell. 2004 Sep 3;118(5):607-17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15339665 15339665] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
Line 21: | Line 30: | ||
[[Category: vps9 domain]] | [[Category: vps9 domain]] | ||
- | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:24:37 2008'' |
Revision as of 12:24, 20 March 2008
| |||||||
, resolution 2.35Å | |||||||
---|---|---|---|---|---|---|---|
Ligands: | |||||||
Gene: | RABGEF1 (Homo sapiens) | ||||||
Coordinates: | save as pdb, mmCIF, xml |
Crystal Structure of the Vps9 Domain of Rabex-5
Overview
The Rab5 GTPase, an essential regulator of endocytosis and endosome biogenesis, is activated by guanine-nucleotide exchange factors (GEFs) that contain a Vps9 domain. Here, we show that the catalytic core of the Rab GEF Rabex-5 has a tandem architecture consisting of a Vps9 domain stabilized by an indispensable helical bundle. A family-wide analysis of Rab specificity demonstrates high selectivity for Rab5 subfamily GTPases. Conserved exchange determinants map to a common surface of the Vps9 domain, which recognizes invariant aromatic residues in the switch regions of Rab GTPases and selects for the Rab5 subfamily by requiring a small nonacidic residue preceding a critical phenylalanine in the switch I region. These and other observations reveal unexpected similarity with the Arf exchange site in the Sec7 domain.
About this Structure
1TXU is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure, exchange determinants, and family-wide rab specificity of the tandem helical bundle and Vps9 domains of Rabex-5., Delprato A, Merithew E, Lambright DG, Cell. 2004 Sep 3;118(5):607-17. PMID:15339665
Page seeded by OCA on Thu Mar 20 14:24:37 2008