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1u8v
From Proteopedia
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| - | [[Image:1u8v.jpg|left|200px]] | + | [[Image:1u8v.jpg|left|200px]] |
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| - | '''Crystal Structure of 4-Hydroxybutyryl-CoA Dehydratase from Clostridium aminobutyricum: Radical catalysis involving a [4Fe-4S] cluster and flavin''' | + | {{Structure |
| + | |PDB= 1u8v |SIZE=350|CAPTION= <scene name='initialview01'>1u8v</scene>, resolution 1.6Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene> and <scene name='pdbligand=FAD:FLAVIN-ADENINE DINUCLEOTIDE'>FAD</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of 4-Hydroxybutyryl-CoA Dehydratase from Clostridium aminobutyricum: Radical catalysis involving a [4Fe-4S] cluster and flavin''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1U8V is a [ | + | 1U8V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_aminobutyricum Clostridium aminobutyricum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U8V OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of 4-hydroxybutyryl-CoA dehydratase: radical catalysis involving a [4Fe-4S] cluster and flavin., Martins BM, Dobbek H, Cinkaya I, Buckel W, Messerschmidt A, Proc Natl Acad Sci U S A. 2004 Nov 2;101(44):15645-9. Epub 2004 Oct 20. PMID:[http:// | + | Crystal structure of 4-hydroxybutyryl-CoA dehydratase: radical catalysis involving a [4Fe-4S] cluster and flavin., Martins BM, Dobbek H, Cinkaya I, Buckel W, Messerschmidt A, Proc Natl Acad Sci U S A. 2004 Nov 2;101(44):15645-9. Epub 2004 Oct 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15496473 15496473] |
[[Category: Clostridium aminobutyricum]] | [[Category: Clostridium aminobutyricum]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: FAD]] | [[Category: FAD]] | ||
[[Category: SF4]] | [[Category: SF4]] | ||
| - | [[Category: alfa- | + | [[Category: alfa-helix]] |
| - | [[Category: beta- | + | [[Category: beta-strand]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:28:50 2008'' |
Revision as of 12:28, 20 March 2008
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| , resolution 1.6Å | |||||||
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| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of 4-Hydroxybutyryl-CoA Dehydratase from Clostridium aminobutyricum: Radical catalysis involving a [4Fe-4S] cluster and flavin
Overview
Dehydratases catalyze the breakage of a carbon-oxygen bond leading to unsaturated products via the elimination of water. The 1.6-A resolution crystal structure of 4-hydroxybutyryl-CoA dehydratase from the gamma-aminobutyrate-fermenting Clostridium aminobutyricum represents a new class of dehydratases with an unprecedented active site architecture. A [4Fe-4S](2+) cluster, coordinated by three cysteine and one histidine residues, is located 7 A from the Re-side of a flavin adenine dinucleotide (FAD) moiety. The structure provides insight into the function of these ubiquitous prosthetic groups in the chemically nonfacile, radical-mediated dehydration of 4-hydroxybutyryl-CoA. The substrate can be bound between the [4Fe-4S](2+) cluster and the FAD with both cofactors contributing to its radical activation and catalytic conversion. Our results raise interesting questions regarding the mechanism of acyl-CoA dehydrogenases, which are involved in fatty acid oxidation, and address the divergent evolution of the ancestral common gene.
About this Structure
1U8V is a Single protein structure of sequence from Clostridium aminobutyricum. Full crystallographic information is available from OCA.
Reference
Crystal structure of 4-hydroxybutyryl-CoA dehydratase: radical catalysis involving a [4Fe-4S] cluster and flavin., Martins BM, Dobbek H, Cinkaya I, Buckel W, Messerschmidt A, Proc Natl Acad Sci U S A. 2004 Nov 2;101(44):15645-9. Epub 2004 Oct 20. PMID:15496473
Page seeded by OCA on Thu Mar 20 14:28:50 2008
