1u8x

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[[Image:1u8x.gif|left|200px]]<br /><applet load="1u8x" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1u8x.gif|left|200px]]
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caption="1u8x, resolution 2.05&Aring;" />
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'''CRYSTAL STRUCTURE OF GLVA FROM BACILLUS SUBTILIS, A METAL-REQUIRING, NAD-DEPENDENT 6-PHOSPHO-ALPHA-GLUCOSIDASE'''<br />
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{{Structure
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|PDB= 1u8x |SIZE=350|CAPTION= <scene name='initialview01'>1u8x</scene>, resolution 2.05&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=G6P:ALPHA-D-GLUCOSE-6-PHOSPHATE'>G6P</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> and <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Maltose-6'-phosphate_glucosidase Maltose-6'-phosphate glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.122 3.2.1.122]
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|GENE= glvA, glvG, glv-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])
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}}
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'''CRYSTAL STRUCTURE OF GLVA FROM BACILLUS SUBTILIS, A METAL-REQUIRING, NAD-DEPENDENT 6-PHOSPHO-ALPHA-GLUCOSIDASE'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1U8X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with <scene name='pdbligand=G6P:'>G6P</scene>, <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=NAD:'>NAD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. This structure supersedes the now removed PDB entry 1NRH. Active as [http://en.wikipedia.org/wiki/Maltose-6'-phosphate_glucosidase Maltose-6'-phosphate glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.122 3.2.1.122] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U8X OCA].
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1U8X is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. This structure supersedes the now removed PDB entry 1NRH. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U8X OCA].
==Reference==
==Reference==
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Novel catalytic mechanism of glycoside hydrolysis based on the structure of an NAD+/Mn2+ -dependent phospho-alpha-glucosidase from Bacillus subtilis., Rajan SS, Yang X, Collart F, Yip VL, Withers SG, Varrot A, Thompson J, Davies GJ, Anderson WF, Structure. 2004 Sep;12(9):1619-29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15341727 15341727]
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Novel catalytic mechanism of glycoside hydrolysis based on the structure of an NAD+/Mn2+ -dependent phospho-alpha-glucosidase from Bacillus subtilis., Rajan SS, Yang X, Collart F, Yip VL, Withers SG, Varrot A, Thompson J, Davies GJ, Anderson WF, Structure. 2004 Sep;12(9):1619-29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15341727 15341727]
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
[[Category: Maltose-6'-phosphate glucosidase]]
[[Category: Maltose-6'-phosphate glucosidase]]
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[[Category: glucosidase]]
[[Category: glucosidase]]
[[Category: mcsg]]
[[Category: mcsg]]
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[[Category: midwest center for structural genomics]]
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[[Category: midwest center for structural genomic]]
[[Category: nad-dependent]]
[[Category: nad-dependent]]
[[Category: protein structure initiative]]
[[Category: protein structure initiative]]
[[Category: psi]]
[[Category: psi]]
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[[Category: structural genomics]]
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[[Category: structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:21:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:28:46 2008''

Revision as of 12:28, 20 March 2008

Image:1u8x.gif


PDB ID 1u8x

Drag the structure with the mouse to rotate
, resolution 2.05Å
Ligands: , and
Gene: glvA, glvG, glv-1 (Bacillus subtilis)
Activity: Maltose-6'-phosphate glucosidase, with EC number 3.2.1.122
Coordinates: save as pdb, mmCIF, xml



CRYSTAL STRUCTURE OF GLVA FROM BACILLUS SUBTILIS, A METAL-REQUIRING, NAD-DEPENDENT 6-PHOSPHO-ALPHA-GLUCOSIDASE


Overview

GlvA, a 6-phospho-alpha-glucosidase from Bacillus subtilis, catalyzes the hydrolysis of maltose-6'-phosphate and belongs to glycoside hydrolase family GH4. GH4 enzymes are unique in their requirement for NAD(H) and a divalent metal for activity. We have determined the crystal structure of GlvA in complex with its ligands to 2.05 A resolution. Analyses of the active site architecture, in conjunction with mechanistic studies and precedent from the nucleotide diphosphate hexose dehydratases and other systems, suggest a novel mechanism of glycoside hydrolysis by GlvA that involves both the NAD(H) and the metal.

About this Structure

1U8X is a Single protein structure of sequence from Bacillus subtilis. This structure supersedes the now removed PDB entry 1NRH. Full crystallographic information is available from OCA.

Reference

Novel catalytic mechanism of glycoside hydrolysis based on the structure of an NAD+/Mn2+ -dependent phospho-alpha-glucosidase from Bacillus subtilis., Rajan SS, Yang X, Collart F, Yip VL, Withers SG, Varrot A, Thompson J, Davies GJ, Anderson WF, Structure. 2004 Sep;12(9):1619-29. PMID:15341727

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