1uas

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[[Image:1uas.jpg|left|200px]]<br /><applet load="1uas" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1uas.jpg|left|200px]]
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caption="1uas, resolution 1.50&Aring;" />
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'''Crystal structure of rice alpha-galactosidase'''<br />
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{{Structure
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|PDB= 1uas |SIZE=350|CAPTION= <scene name='initialview01'>1uas</scene>, resolution 1.50&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=PT:PLATINUM+(II)+ION'>PT</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Alpha-galactosidase Alpha-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.22 3.2.1.22]
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|GENE=
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}}
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'''Crystal structure of rice alpha-galactosidase'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1UAS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Oryza_sativa Oryza sativa] with <scene name='pdbligand=GLA:'>GLA</scene>, <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=PT:'>PT</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alpha-galactosidase Alpha-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.22 3.2.1.22] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UAS OCA].
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1UAS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Oryza_sativa Oryza sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UAS OCA].
==Reference==
==Reference==
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Crystal structure of rice alpha-galactosidase complexed with D-galactose., Fujimoto Z, Kaneko S, Momma M, Kobayashi H, Mizuno H, J Biol Chem. 2003 May 30;278(22):20313-8. Epub 2003 Mar 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12657636 12657636]
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Crystal structure of rice alpha-galactosidase complexed with D-galactose., Fujimoto Z, Kaneko S, Momma M, Kobayashi H, Mizuno H, J Biol Chem. 2003 May 30;278(22):20313-8. Epub 2003 Mar 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12657636 12657636]
[[Category: Alpha-galactosidase]]
[[Category: Alpha-galactosidase]]
[[Category: Oryza sativa]]
[[Category: Oryza sativa]]
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[[Category: tim-barrel]]
[[Category: tim-barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:22:27 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:29:31 2008''

Revision as of 12:29, 20 March 2008

Image:1uas.jpg


PDB ID 1uas

Drag the structure with the mouse to rotate
, resolution 1.50Å
Ligands: , , and
Activity: Alpha-galactosidase, with EC number 3.2.1.22
Coordinates: save as pdb, mmCIF, xml



Crystal structure of rice alpha-galactosidase


Overview

alpha-Galactosidases catalyze the hydrolysis of alpha-1,6-linked galactosyl residues from galacto-oligosaccharides and polymeric galacto-(gluco)mannans. The crystal structure of rice alpha-galactosidase has been determined at 1.5A resolution using the multiple isomorphous replacement method. The structure consisted of a catalytic domain and a C-terminal domain and was essentially the same as that of alpha-N-acetylgalactosaminidase, which is the same member of glycosyl hydrolase family 27. The catalytic domain had a (beta/alpha)8-barrel structure, and the C-terminal domain was made up of eight beta-strands containing a Greek key motif. The structure was solved as a complex with d-galactose, providing a mode of substrate binding in detail. The d-galactose molecule was found bound in the active site pocket on the C-terminal side of the central beta-barrel of the catalytic domain. The d-galactose molecule consisted of a mixture of two anomers present in a ratio equal to their natural abundance. Structural comparisons of rice alpha-galactosidase with chicken alpha-N-acetylgalactosaminidase provided further understanding of the substrate recognition mechanism in these enzymes.

About this Structure

1UAS is a Single protein structure of sequence from Oryza sativa. Full crystallographic information is available from OCA.

Reference

Crystal structure of rice alpha-galactosidase complexed with D-galactose., Fujimoto Z, Kaneko S, Momma M, Kobayashi H, Mizuno H, J Biol Chem. 2003 May 30;278(22):20313-8. Epub 2003 Mar 25. PMID:12657636

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