1ubi
From Proteopedia
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| - | [[Image:1ubi.jpg|left|200px]] | + | [[Image:1ubi.jpg|left|200px]] |
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| - | '''SYNTHETIC STRUCTURAL AND BIOLOGICAL STUDIES OF THE UBIQUITIN SYSTEM. PART 1''' | + | {{Structure |
| + | |PDB= 1ubi |SIZE=350|CAPTION= <scene name='initialview01'>1ubi</scene>, resolution 1.8Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''SYNTHETIC STRUCTURAL AND BIOLOGICAL STUDIES OF THE UBIQUITIN SYSTEM. PART 1''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1UBI is a [ | + | 1UBI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UBI OCA]. |
==Reference== | ==Reference== | ||
| - | Synthetic, structural and biological studies of the ubiquitin system: the total chemical synthesis of ubiquitin., Ramage R, Green J, Muir TW, Ogunjobi OM, Love S, Shaw K, Biochem J. 1994 Apr 1;299 ( Pt 1):151-8. PMID:[http:// | + | Synthetic, structural and biological studies of the ubiquitin system: the total chemical synthesis of ubiquitin., Ramage R, Green J, Muir TW, Ogunjobi OM, Love S, Shaw K, Biochem J. 1994 Apr 1;299 ( Pt 1):151-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8166633 8166633] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: chromosomal protein]] | [[Category: chromosomal protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:29:48 2008'' |
Revision as of 12:29, 20 March 2008
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| , resolution 1.8Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
SYNTHETIC STRUCTURAL AND BIOLOGICAL STUDIES OF THE UBIQUITIN SYSTEM. PART 1
Contents |
Overview
The small protein ubiquitin (76 amino acids) has been synthesized under optimized conditions by Merrifield solid-phase methodology using the N alpha-Fmoc protecting group. The crude polypeptide mixture was purified to homogeneity by gel filtration, dialysis and a combination of cation- and anion-exchange chromatography to yield ubiquitin. Amino acid analysis, enzymic digestion and sequencing by automated Edman degradation were used to authenticate the primary structure. Isoelectric focusing and m.s. were used to demonstrate that the final product was greater than 98% pure with a final yield of 93 mg (4.3%) from a single synthesis on a 0.25 nmol scale.
Disease
Known disease associated with this structure: Cleft palate, isolated OMIM:[191339]
About this Structure
1UBI is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Synthetic, structural and biological studies of the ubiquitin system: the total chemical synthesis of ubiquitin., Ramage R, Green J, Muir TW, Ogunjobi OM, Love S, Shaw K, Biochem J. 1994 Apr 1;299 ( Pt 1):151-8. PMID:8166633
Page seeded by OCA on Thu Mar 20 14:29:48 2008
