1wmw
From Proteopedia
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- | [[ | + | ==Crystal structure of geranulgeranyl diphosphate synthase from Thermus thermophilus== |
+ | <StructureSection load='1wmw' size='340' side='right' caption='[[1wmw]], [[Resolution|resolution]] 1.55Å' scene=''> | ||
+ | == Structural highlights == | ||
+ | <table><tr><td colspan='2'>[[1wmw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb8 Thermus thermophilus hb8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WMW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WMW FirstGlance]. <br> | ||
+ | </td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wmw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wmw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1wmw RCSB], [http://www.ebi.ac.uk/pdbsum/1wmw PDBsum], [http://www.topsan.org/Proteins/RSGI/1wmw TOPSAN]</span></td></tr> | ||
+ | <table> | ||
+ | == Evolutionary Conservation == | ||
+ | [[Image:Consurf_key_small.gif|200px|right]] | ||
+ | Check<jmol> | ||
+ | <jmolCheckbox> | ||
+ | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wm/1wmw_consurf.spt"</scriptWhenChecked> | ||
+ | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
+ | <text>to colour the structure by Evolutionary Conservation</text> | ||
+ | </jmolCheckbox> | ||
+ | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | ||
+ | <div style="clear:both"></div> | ||
+ | <div style="background-color:#fffaf0;"> | ||
+ | == Publication Abstract from PubMed == | ||
+ | Geranylgeranyl diphosphate (GGPP) synthase from Thermus thermophilus HB8 was expressed in Escherichia coli, purified to homogeneity and crystallized both as the recombinant native protein and its selenomethionine (SeMet) derivative. Well diffracting crystals of these proteins were obtained belonging to the tetragonal space group P4(1) or P4(3), with unit-cell parameters a = b = 139.88, c = 73.37 A. There were two homodimers in the asymmetric unit. A native data set was collected to 1.55 A resolution and a data set suitable for MAD phasing was collected to 2.40 A resolution on beamline BL40B2 at SPring-8. | ||
- | + | Expression, purification, crystallization and preliminary X-ray studies of geranylgeranyl diphosphate synthase from Thermus thermophilus HB8.,Nishio K, Nodake Y, Hamada K, Suto K, Nakagawa N, Kuramitsu S, Miura K Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):178-80. Epub 2003, Dec 18. PMID:14684922<ref>PMID:14684922</ref> | |
- | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
- | + | </div> | |
- | + | == References == | |
- | + | <references/> | |
- | + | __TOC__ | |
- | + | </StructureSection> | |
- | + | ||
- | == | + | |
- | < | + | |
[[Category: Thermus thermophilus hb8]] | [[Category: Thermus thermophilus hb8]] | ||
[[Category: Hamada, K.]] | [[Category: Hamada, K.]] |
Revision as of 19:54, 29 September 2014
Crystal structure of geranulgeranyl diphosphate synthase from Thermus thermophilus
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Categories: Thermus thermophilus hb8 | Hamada, K. | Kawamoto, M. | Kuramitu, S. | Miura, K. | Nakagawa, N. | Nishio, K. | Nodake, Y. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Suto, K. | Ggpp | Prenyl diphosphate synthase | Riken structural genomics/proteomics initiative | Rsgi | Structural genomic | Thermus | Transferase