1ue4

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1ue4.gif|left|200px]]<br /><applet load="1ue4" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:1ue4.gif|left|200px]]
-
caption="1ue4, resolution 1.65&Aring;" />
+
 
-
'''Crystal structure of d(GCGAAAGC)'''<br />
+
{{Structure
 +
|PDB= 1ue4 |SIZE=350|CAPTION= <scene name='initialview01'>1ue4</scene>, resolution 1.65&Aring;
 +
|SITE=
 +
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene>
 +
|ACTIVITY=
 +
|GENE=
 +
}}
 +
 
 +
'''Crystal structure of d(GCGAAAGC)'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
1UE4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UE4 OCA].
+
1UE4 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UE4 OCA].
==Reference==
==Reference==
-
Structures of d(GCGAAGC) and d(GCGAAAGC) (tetragonal form): a switching of partners of the sheared G.A pairs to form a functional G.AxA.G crossing., Sunami T, Kondo J, Hirao I, Watanabe K, Miura K, Takenaka A, Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):422-31. Epub 2004, Feb 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14993665 14993665]
+
Structures of d(GCGAAGC) and d(GCGAAAGC) (tetragonal form): a switching of partners of the sheared G.A pairs to form a functional G.AxA.G crossing., Sunami T, Kondo J, Hirao I, Watanabe K, Miura K, Takenaka A, Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):422-31. Epub 2004, Feb 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14993665 14993665]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Hirao, I.]]
[[Category: Hirao, I.]]
Line 24: Line 33:
[[Category: sheared g:a pair]]
[[Category: sheared g:a pair]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:23:28 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:30:49 2008''

Revision as of 12:30, 20 March 2008

Image:1ue4.gif


PDB ID 1ue4

Drag the structure with the mouse to rotate
, resolution 1.65Å
Ligands:
Coordinates: save as pdb, mmCIF, xml



Crystal structure of d(GCGAAAGC)


Overview

The DNA fragments d(GCGAAGC) and d(GCGAAAGC) are known to exhibit several extraordinary properties. Their crystal structures have been determined at 1.6 and 1.65 A resolution, respectively. Two heptamers aligned in an antiparallel fashion associate to form a duplex having molecular twofold symmetry. In the crystallographic asymmetric unit, there are three structurally identical duplexes. At both ends of each duplex, two Watson-Crick G.C pairs constitute the stem regions. In the central part, two sheared G.A pairs are crossed and stacked on each other, so that the stacked two guanine bases of the G.AxA.G crossing expose their Watson-Crick and major-groove sites into solvent, suggesting a functional role. The adenine moieties of the A(5) residues are inside the duplex, wedged between the A(4) and G(6) residues, but there are no partners for interactions. To close the open space on the counter strand, the duplex is strongly bent. In the asymmetric unit of the d(GCGAAAGC) crystal (tetragonal form), there is only one octamer chain. However, the two chains related by the crystallographic twofold symmetry associate to form an antiparallel duplex, similar to the base-intercalated duplex found in the hexagonal crystal form of the octamer. It is interesting to note that the significant difference between the present bulge-in structure of d(GCGAAGC) and the base-intercalated duplex of d(GCGAAAGC) can be ascribed to a switching of partners of the sheared G.A pairs.

About this Structure

1UE4 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

Structures of d(GCGAAGC) and d(GCGAAAGC) (tetragonal form): a switching of partners of the sheared G.A pairs to form a functional G.AxA.G crossing., Sunami T, Kondo J, Hirao I, Watanabe K, Miura K, Takenaka A, Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):422-31. Epub 2004, Feb 25. PMID:14993665

Page seeded by OCA on Thu Mar 20 14:30:49 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ue4"
Personal tools