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1umh

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[[Image:1umh.gif|left|200px]]<br /><applet load="1umh" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1umh.gif|left|200px]]
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caption="1umh, resolution 2.00&Aring;" />
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'''Structural basis of sugar-recognizing ubiquitin ligase'''<br />
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{{Structure
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|PDB= 1umh |SIZE=350|CAPTION= <scene name='initialview01'>1umh</scene>, resolution 2.00&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=NI:NICKEL (II) ION'>NI</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19]
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|GENE= mouse ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])
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}}
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'''Structural basis of sugar-recognizing ubiquitin ligase'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1UMH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=NI:'>NI</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UMH OCA].
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1UMH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UMH OCA].
==Reference==
==Reference==
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Structural basis of sugar-recognizing ubiquitin ligase., Mizushima T, Hirao T, Yoshida Y, Lee SJ, Chiba T, Iwai K, Yamaguchi Y, Kato K, Tsukihara T, Tanaka K, Nat Struct Mol Biol. 2004 Apr;11(4):365-70. Epub 2004 Feb 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14990996 14990996]
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Structural basis of sugar-recognizing ubiquitin ligase., Mizushima T, Hirao T, Yoshida Y, Lee SJ, Chiba T, Iwai K, Yamaguchi Y, Kato K, Tsukihara T, Tanaka K, Nat Struct Mol Biol. 2004 Apr;11(4):365-70. Epub 2004 Feb 29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14990996 14990996]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: rsgi]]
[[Category: rsgi]]
[[Category: scf]]
[[Category: scf]]
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[[Category: structural genomics]]
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[[Category: structural genomic]]
[[Category: ubiquitin]]
[[Category: ubiquitin]]
[[Category: ubiquitin ligase]]
[[Category: ubiquitin ligase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:26:05 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:33:56 2008''

Revision as of 12:33, 20 March 2008

Image:1umh.gif


PDB ID 1umh

Drag the structure with the mouse to rotate
, resolution 2.00Å
Ligands:
Gene: mouse (Mus musculus)
Activity: Ubiquitin--protein ligase, with EC number 6.3.2.19
Coordinates: save as pdb, mmCIF, xml



Structural basis of sugar-recognizing ubiquitin ligase


Overview

SCF(Fbs1) is a ubiquitin ligase that functions in the endoplasmic reticulum (ER)-associated degradation pathway. Fbs1/Fbx2, a member of the F-box proteins, recognizes high-mannose oligosaccharides. Efficient binding to an N-glycan requires di-N-acetylchitobiose (chitobiose). Here we report the crystal structures of the sugar-binding domain (SBD) of Fbs1 alone and in complex with chitobiose. The SBD is composed of a ten-stranded antiparallel beta-sandwich. The structure of the SBD-chitobiose complex includes hydrogen bonds between Fbs1 and chitobiose and insertion of the methyl group of chitobiose into a small hydrophobic pocket of Fbs1. Moreover, NMR spectroscopy has demonstrated that the amino acid residues adjoining the chitobiose-binding site interact with the outer branches of the carbohydrate moiety. Considering that the innermost chitobiose moieties in N-glycans are usually involved in intramolecular interactions with the polypeptide moieties, we propose that Fbs1 interacts with the chitobiose in unfolded N-glycoprotein, pointing the protein moiety toward E2 for ubiquitination.

About this Structure

1UMH is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

Structural basis of sugar-recognizing ubiquitin ligase., Mizushima T, Hirao T, Yoshida Y, Lee SJ, Chiba T, Iwai K, Yamaguchi Y, Kato K, Tsukihara T, Tanaka K, Nat Struct Mol Biol. 2004 Apr;11(4):365-70. Epub 2004 Feb 29. PMID:14990996

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