1ush
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1ush.jpg|left|200px]] | + | [[Image:1ush.jpg|left|200px]] |
| - | + | ||
| - | '''5'-NUCLEOTIDASE FROM E. COLI''' | + | {{Structure |
| + | |PDB= 1ush |SIZE=350|CAPTION= <scene name='initialview01'>1ush</scene>, resolution 1.73Å | ||
| + | |SITE= <scene name='pdbsite=ZNB:Zn+Binding+Site'>ZNB</scene> | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=CO3:CARBONATE+ION'>CO3</scene> and <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/5'-nucleotidase 5'-nucleotidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.5 3.1.3.5] | ||
| + | |GENE= USHA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | }} | ||
| + | |||
| + | '''5'-NUCLEOTIDASE FROM E. COLI''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1USH is a [ | + | 1USH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1USH OCA]. |
==Reference== | ==Reference== | ||
| - | X-ray structure of the Escherichia coli periplasmic 5'-nucleotidase containing a dimetal catalytic site., Knofel T, Strater N, Nat Struct Biol. 1999 May;6(5):448-53. PMID:[http:// | + | X-ray structure of the Escherichia coli periplasmic 5'-nucleotidase containing a dimetal catalytic site., Knofel T, Strater N, Nat Struct Biol. 1999 May;6(5):448-53. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10331872 10331872] |
[[Category: 5'-nucleotidase]] | [[Category: 5'-nucleotidase]] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
| Line 24: | Line 33: | ||
[[Category: udp-sugar hydrolase]] | [[Category: udp-sugar hydrolase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:36:08 2008'' |
Revision as of 12:36, 20 March 2008
| |||||||
| , resolution 1.73Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | |||||||
| Ligands: | , and | ||||||
| Gene: | USHA (Escherichia coli) | ||||||
| Activity: | 5'-nucleotidase, with EC number 3.1.3.5 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
5'-NUCLEOTIDASE FROM E. COLI
Overview
The crystal structure of 5'-nucleotidase (5'-NT) from E. coli, also known as UDP-sugar hydrolase, has been determined at 1.7 A resolution. Two zinc ions are present in the active site, which is located in a cleft between two domains. The dimetal center and a catalytic Asp-His dyad are the main players in the catalytic mechanism. Structure-based sequence comparisons show that the structure also provides a model for animal 5'-NTs, which together with other ectonucleotidases terminate the action of nucleotides as extracellular signaling substances in the nervous system.
About this Structure
1USH is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
X-ray structure of the Escherichia coli periplasmic 5'-nucleotidase containing a dimetal catalytic site., Knofel T, Strater N, Nat Struct Biol. 1999 May;6(5):448-53. PMID:10331872
Page seeded by OCA on Thu Mar 20 14:36:08 2008
