1uzz

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[[Image:1uzz.gif|left|200px]]<br /><applet load="1uzz" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1uzz.gif|left|200px]]
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caption="1uzz, resolution 2.13&Aring;" />
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'''ERYTHRINA CRISTAGALLI BOUND TO N-LINKED OLIGOSACCHARIDE AND LACTOSE'''<br />
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{{Structure
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|PDB= 1uzz |SIZE=350|CAPTION= <scene name='initialview01'>1uzz</scene>, resolution 2.13&Aring;
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|SITE= <scene name='pdbsite=AC1:Mn+Binding+Site+For+Chain+D'>AC1</scene>
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''ERYTHRINA CRISTAGALLI BOUND TO N-LINKED OLIGOSACCHARIDE AND LACTOSE'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1UZZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Eukaryota Eukaryota] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Mn+Binding+Site+For+Chain+D'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UZZ OCA].
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1UZZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Eukaryota Eukaryota]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UZZ OCA].
==Reference==
==Reference==
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Crystal structures of Erythrina cristagalli lectin with bound N-linked oligosaccharide and lactose., Turton K, Natesh R, Thiyagarajan N, Chaddock JA, Acharya KR, Glycobiology. 2004 Oct;14(10):923-9. Epub 2004 Jun 16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15201215 15201215]
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Crystal structures of Erythrina cristagalli lectin with bound N-linked oligosaccharide and lactose., Turton K, Natesh R, Thiyagarajan N, Chaddock JA, Acharya KR, Glycobiology. 2004 Oct;14(10):923-9. Epub 2004 Jun 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15201215 15201215]
[[Category: Eukaryota]]
[[Category: Eukaryota]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: lectin]]
[[Category: lectin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:30:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:39:06 2008''

Revision as of 12:39, 20 March 2008

Image:1uzz.gif


PDB ID 1uzz

Drag the structure with the mouse to rotate
, resolution 2.13Å
Sites:
Ligands: , and
Coordinates: save as pdb, mmCIF, xml



ERYTHRINA CRISTAGALLI BOUND TO N-LINKED OLIGOSACCHARIDE AND LACTOSE


Overview

Erythrina cristagalli lectin (ECL) is a galactose-specific legume lectin. Although its biological function in the legume is unknown, ECL exhibits hemagglutinating activity in vitro and is mitogenic for T lymphocytes. In addition, it has been recently shown that ECL forms a novel conjugate when coupled to a catalytically active derivative of the type A neurotoxin from Clostridium botulinum, thus providing a therapeutic potential. ECL is biologically active as a dimer in which each protomer contains a functional carbohydrate-combining site. The crystal structure of native ECL was recently reported in complex with lactose and 2'-fucosyllactose. ECL protomers adopt the legume lectin fold but form non-canonical dimers via the handshake motif as was previously observed for Erythrina corallodendron lectin. Here we report the crystal structures of native and recombinant forms of the lectin in three new crystal forms, both unliganded and in complex with lactose. For the first time, the detailed structure of the glycosylated hexasaccharide for native ECL has been elucidated. The structure also shows that in the crystal lattice the glycosylation site and the carbohydrate binding site are involved in intermolecular contacts through water-mediated interactions.

About this Structure

1UZZ is a Single protein structure of sequence from Eukaryota. Full crystallographic information is available from OCA.

Reference

Crystal structures of Erythrina cristagalli lectin with bound N-linked oligosaccharide and lactose., Turton K, Natesh R, Thiyagarajan N, Chaddock JA, Acharya KR, Glycobiology. 2004 Oct;14(10):923-9. Epub 2004 Jun 16. PMID:15201215

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